We studied the effect of brain and spinal cord injury induced by fluid-percussion on the local synthesis of neurosteroids as measured by a gas-chromatographic/mass-spectrometric method. In the nervous system of sham operated rats i.v. infusion of pregnenolone (PREGN)-sulfate results in a 2-4 fold increase in PREGN, progesterone (PROG), 5alpha-dehydroprogesterone (5 alpha-DHP) and 3alpha-hydroxy-5alpha-pregnan-20-one (3 alpha 5 alpha-THP, allopregnanolone) concentrations, as compared to vehicle treated rats. When PREGN-sulfate was infused 1,3 or 7 days after brain or spinal cord injury it was observed a large time-dependent increase of FROG, 5 alpha-DHP and 3 alpha 5 alpha-THP levels in the peri-focal but not in the focal site. This increase in neurosteroids content may be due essentially to the glial cells hyperplasia in the peri-focal area and to an activation of the pathways involved in the metabolism of PREGN-sulfate to FROG, 5 alpha-DHP and 3 alpha 5 alpha-THP. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
Di Michele F., L.D. (2000). Increased neurosteroids synthesis after brain and spinal cord injury in rats. NEUROSCIENCE LETTERS, 284, 65-68 [10.1016/S0304-3940(00)00965-4].
Tipologia: | Articolo su rivista | |
Citazione: | Di Michele F., L.D. (2000). Increased neurosteroids synthesis after brain and spinal cord injury in rats. NEUROSCIENCE LETTERS, 284, 65-68 [10.1016/S0304-3940(00)00965-4]. | |
IF: | Con Impact Factor ISI | |
Lingua: | English | |
Settore Scientifico Disciplinare: | Settore BIO/09 Settore MED/39 - Neuropsichiatria Infantile | |
Revisione (peer review): | Sì, ma tipo non specificato | |
Tipo: | Articolo | |
Rilevanza: | Rilevanza internazionale | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0304-3940(00)00965-4 | |
Stato di pubblicazione: | Pubblicato | |
Data di pubblicazione: | 2000 | |
Titolo: | Increased neurosteroids synthesis after brain and spinal cord injury in rats | |
Autori: | ||
Autori: | Di Michele F., Lekieffre D., Pasini A., Bernardi G., Benavides J., Romeo E. | |
Appare nelle tipologie: | 01 - Articolo su rivista |