Locally liberated cytokines and extracellular matrix components influence the proliferation and differentiation of arterial smooth muscle cells (SMC), thus playing a role in the development of the atheromatous plaque. It has been proposed that the response of SMC to these factors is influenced by their own phenotype. We have tested the effects of transforming growth factor-beta 1 (TGF-beta 1) and heparin on proliferation and expression of alpha-smooth muscle (SM) actin, a well-established SMC differentiation marker, by cultured rat SMC obtained from the normal aorta of young or old rats and from the intimal thickening developed 15 days after endothelial denudation in young rats; these SMC are known to express different phenotypic features. Heparin and TGF-beta 1 reduced serum-induced proliferation in SMC from young and old rats. Heparin increased the expression of alpha-SM actin protein and mRNA in SMC from young and old rats, while TGF-beta 1 exerted the opposite action. Moreover, TGF-beta 1 induced the appearance of an elongated shape in SMC from both young and old rats. In SMC cultured from intimal thickening, heparin induced a reduction of cell proliferation without modifying their characteristic epithelioid shape; TGF-beta 1 increased the proliferative activity and induced an elongated cell shape as well as a ''hills and valleys'' growth pattern similar to that observed in control medial SMC; both heparin and TGF-beta 1 induced an increase of alpha-SM actin expression. Our results show that TGF-beta 1 and heparin exert different effects on the same SMC, suggesting that these substances act at least in part independently. They are also compatible with the view that the action of cytokines and of extracellular matrix components depends on the phenotype of target SMC. (C) 1994 Academic Press, Inc.

Orlandi, A., Ropraz, P., & Gabbiani, G. (1994). PROLIFERATIVE ACTIVITY AND ALPHA-SMOOTH MUSCLE ACTIN EXPRESSION IN CULTURED RAT AORTIC SMOOTH-MUSCLE CELLS ARE DIFFERENTLY MODULATED BY TRANSFORMING GROWTH-FACTOR-BETA-1 AND HEPARIN. EXPERIMENTAL CELL RESEARCH, 214(2), 528-536 [10.1006/excr.1994.1290].

PROLIFERATIVE ACTIVITY AND ALPHA-SMOOTH MUSCLE ACTIN EXPRESSION IN CULTURED RAT AORTIC SMOOTH-MUSCLE CELLS ARE DIFFERENTLY MODULATED BY TRANSFORMING GROWTH-FACTOR-BETA-1 AND HEPARIN

ORLANDI, AUGUSTO;
1994

Abstract

Locally liberated cytokines and extracellular matrix components influence the proliferation and differentiation of arterial smooth muscle cells (SMC), thus playing a role in the development of the atheromatous plaque. It has been proposed that the response of SMC to these factors is influenced by their own phenotype. We have tested the effects of transforming growth factor-beta 1 (TGF-beta 1) and heparin on proliferation and expression of alpha-smooth muscle (SM) actin, a well-established SMC differentiation marker, by cultured rat SMC obtained from the normal aorta of young or old rats and from the intimal thickening developed 15 days after endothelial denudation in young rats; these SMC are known to express different phenotypic features. Heparin and TGF-beta 1 reduced serum-induced proliferation in SMC from young and old rats. Heparin increased the expression of alpha-SM actin protein and mRNA in SMC from young and old rats, while TGF-beta 1 exerted the opposite action. Moreover, TGF-beta 1 induced the appearance of an elongated shape in SMC from both young and old rats. In SMC cultured from intimal thickening, heparin induced a reduction of cell proliferation without modifying their characteristic epithelioid shape; TGF-beta 1 increased the proliferative activity and induced an elongated cell shape as well as a ''hills and valleys'' growth pattern similar to that observed in control medial SMC; both heparin and TGF-beta 1 induced an increase of alpha-SM actin expression. Our results show that TGF-beta 1 and heparin exert different effects on the same SMC, suggesting that these substances act at least in part independently. They are also compatible with the view that the action of cytokines and of extracellular matrix components depends on the phenotype of target SMC. (C) 1994 Academic Press, Inc.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/08 - Anatomia Patologica
English
Con Impact Factor ISI
alpha actin; heparin; transforming growth factor beta1; aorta; article; cell differentiation; cell proliferation; controlled study; extracellular matrix; gene expression; nonhuman; priority journal; rat; smooth muscle fiber; vascular smooth muscle; Actins; Age Factors; Animal; Aorta; Cell Division; Cell Size; Comparative Study; Cytoskeleton; Dose-Response Relationship, Drug; Female; Heparin; Muscle, Smooth, Vascular; Rats; Rats, Wistar; RNA, Messenger; Support, Non-U.S. Gov't; Transforming Growth Factor beta; Tunica Intima
Orlandi, A., Ropraz, P., & Gabbiani, G. (1994). PROLIFERATIVE ACTIVITY AND ALPHA-SMOOTH MUSCLE ACTIN EXPRESSION IN CULTURED RAT AORTIC SMOOTH-MUSCLE CELLS ARE DIFFERENTLY MODULATED BY TRANSFORMING GROWTH-FACTOR-BETA-1 AND HEPARIN. EXPERIMENTAL CELL RESEARCH, 214(2), 528-536 [10.1006/excr.1994.1290].
Orlandi, A; Ropraz, P; Gabbiani, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/49934
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