Restriction endonucleases have been shown to induce chromosome damage in a variety of cultured cells. We recently reported the coincidence between MspI-induced breakage and the location of common fragile sites. We have extended our study to HpaII, which induced a 4.5-fold increase in total breakage compared to controls. It appeared that a major contribution was given by stable chromosome rearrangements, which were present at a 14-fold increased frequency in comparison to the spontaneous levels. Moreover, several chromosome bands were involved in rearrangements in different cultures from different donors. Notably, HpaII-induced breakage occurred in the same bands where breakpoints of constitutional and neoplastic rearrengements are located.
Tedeschi B., P.B. (1991). Structural chromosomal rearrangements in HpaII-treated human lymphocytes, 248(1), 115-121 [10.1016/0027-5107(91)90093-4].
|Tipologia:||Articolo su rivista|
|Citazione:||Tedeschi B., P.B. (1991). Structural chromosomal rearrangements in HpaII-treated human lymphocytes, 248(1), 115-121 [10.1016/0027-5107(91)90093-4].|
|Settore Scientifico Disciplinare:||Settore BIO/13|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0027-5107(91)90093-4|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||1991|
|Titolo:||Structural chromosomal rearrangements in HpaII-treated human lymphocytes|
|Autori:||Tedeschi B., Porfirio B., Caporossi D., Vernole P., Nicoletti B.|
|Appare nelle tipologie:||01 - Articolo su rivista|