Restriction endonucleases have been shown to induce chromosome damage in a variety of cultured cells. We recently reported the coincidence between MspI-induced breakage and the location of common fragile sites. We have extended our study to HpaII, which induced a 4.5-fold increase in total breakage compared to controls. It appeared that a major contribution was given by stable chromosome rearrangements, which were present at a 14-fold increased frequency in comparison to the spontaneous levels. Moreover, several chromosome bands were involved in rearrangements in different cultures from different donors. Notably, HpaII-induced breakage occurred in the same bands where breakpoints of constitutional and neoplastic rearrengements are located.

Tedeschi B., P.B. (1991). Structural chromosomal rearrangements in HpaII-treated human lymphocytes, 248(1), 115-121 [10.1016/0027-5107(91)90093-4].

Structural chromosomal rearrangements in HpaII-treated human lymphocytes

VERNOLE, PATRIZIA;
1991

Abstract

Restriction endonucleases have been shown to induce chromosome damage in a variety of cultured cells. We recently reported the coincidence between MspI-induced breakage and the location of common fragile sites. We have extended our study to HpaII, which induced a 4.5-fold increase in total breakage compared to controls. It appeared that a major contribution was given by stable chromosome rearrangements, which were present at a 14-fold increased frequency in comparison to the spontaneous levels. Moreover, several chromosome bands were involved in rearrangements in different cultures from different donors. Notably, HpaII-induced breakage occurred in the same bands where breakpoints of constitutional and neoplastic rearrengements are located.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/13
English
restriction endonuclease; article; chromosome breakage; chromosome damage; chromosome fragile site; human; human cell; lymphocyte culture; male; normal human; priority journal; Deoxyribonuclease HpaII; Deoxyribonucleases, Type II Site-Specific; DNA; DNA Mutational Analysis; Gene Rearrangement; Human; In Vitro; Karyotyping; Lymphocytes; Polymorphism, Restriction Fragment Length; Support, Non-U.S. Gov't
Tedeschi B., P.B. (1991). Structural chromosomal rearrangements in HpaII-treated human lymphocytes, 248(1), 115-121 [10.1016/0027-5107(91)90093-4].
Tedeschi, B; Porfirio, B; Caporossi, D; Vernole, P; Nicoletti, B
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/49923
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