Restriction endonucleases have been shown to induce chromosome damage in a variety of cultured cells. We recently reported the coincidence between MspI-induced breakage and the location of common fragile sites. We have extended our study to HpaII, which induced a 4.5-fold increase in total breakage compared to controls. It appeared that a major contribution was given by stable chromosome rearrangements, which were present at a 14-fold increased frequency in comparison to the spontaneous levels. Moreover, several chromosome bands were involved in rearrangements in different cultures from different donors. Notably, HpaII-induced breakage occurred in the same bands where breakpoints of constitutional and neoplastic rearrengements are located.
Tedeschi B., P.B. (1991). Structural chromosomal rearrangements in HpaII-treated human lymphocytes, 248(1), 115-121.
Tipologia: | Articolo su rivista |
Citazione: | Tedeschi B., P.B. (1991). Structural chromosomal rearrangements in HpaII-treated human lymphocytes, 248(1), 115-121. |
Lingua: | English |
Settore Scientifico Disciplinare: | Settore BIO/13 |
Revisione (peer review): | Sì, ma tipo non specificato |
Tipo: | Articolo |
Rilevanza: | Rilevanza internazionale |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/0027-5107(91)90093-4 |
Stato di pubblicazione: | Pubblicato |
Data di pubblicazione: | 1991 |
Titolo: | Structural chromosomal rearrangements in HpaII-treated human lymphocytes |
Autori: | |
Autori: | Tedeschi B., Porfirio B., Caporossi D., Vernole P., Nicoletti B. |
Appare nelle tipologie: | 01 - Articolo su rivista |