Restriction endonucleases have been shown to induce chromosome damage in a variety of cultured cells. We recently reported the coincidence between MspI-induced breakage and the location of common fragile sites. We have extended our study to HpaII, which induced a 4.5-fold increase in total breakage compared to controls. It appeared that a major contribution was given by stable chromosome rearrangements, which were present at a 14-fold increased frequency in comparison to the spontaneous levels. Moreover, several chromosome bands were involved in rearrangements in different cultures from different donors. Notably, HpaII-induced breakage occurred in the same bands where breakpoints of constitutional and neoplastic rearrengements are located.
Tedeschi, B., Porfirio, B., Caporossi, D., Vernole, P., Nicoletti, B. (1991). Structural chromosomal rearrangements in HpaII-treated human lymphocytes, 248(1), 115-121 [10.1016/0027-5107(91)90093-4].
Structural chromosomal rearrangements in HpaII-treated human lymphocytes
VERNOLE, PATRIZIA;
1991-01-01
Abstract
Restriction endonucleases have been shown to induce chromosome damage in a variety of cultured cells. We recently reported the coincidence between MspI-induced breakage and the location of common fragile sites. We have extended our study to HpaII, which induced a 4.5-fold increase in total breakage compared to controls. It appeared that a major contribution was given by stable chromosome rearrangements, which were present at a 14-fold increased frequency in comparison to the spontaneous levels. Moreover, several chromosome bands were involved in rearrangements in different cultures from different donors. Notably, HpaII-induced breakage occurred in the same bands where breakpoints of constitutional and neoplastic rearrengements are located.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.