This paper describes an electrochemical biosensor for free cholesterol monitoring. The sensor is a multienzymatic electrodic system in which horseradish peroxidase and cholesterol oxidase are simultaneously immobilized within a polymeric film. on the surface of a pyrolitic graphite electrode. From voltammetric and amperometric (flow-injection) data obtained, the efficiency, reproducibility and stability of the system are discussed. Results obtained, of interest for basic and applied biochemistry, represent a first step for construction of a mediator-free biosensor with potentialities for a successful application in the biosensor area. (C) 2001 Elsevier Science BN. All rights reserved.

Bongiovanni C., F.T. (2001). An electrochemical multienzymatic biosensor for determination of cholesterol. BIOELECTROCHEMISTRY, 54(1), 17-22.

An electrochemical multienzymatic biosensor for determination of cholesterol

SANTUCCI, ROBERTO;DESIDERI, ALESSANDRO
2001

Abstract

This paper describes an electrochemical biosensor for free cholesterol monitoring. The sensor is a multienzymatic electrodic system in which horseradish peroxidase and cholesterol oxidase are simultaneously immobilized within a polymeric film. on the surface of a pyrolitic graphite electrode. From voltammetric and amperometric (flow-injection) data obtained, the efficiency, reproducibility and stability of the system are discussed. Results obtained, of interest for basic and applied biochemistry, represent a first step for construction of a mediator-free biosensor with potentialities for a successful application in the biosensor area. (C) 2001 Elsevier Science BN. All rights reserved.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10
English
Con Impact Factor ISI
Biosensor; Cholesterol; Cyclic voltammetry; Flow injection analysis; Horseradish peroxidase
Bongiovanni C., F.T. (2001). An electrochemical multienzymatic biosensor for determination of cholesterol. BIOELECTROCHEMISTRY, 54(1), 17-22.
Bongiovanni, C; Ferri, T; Poscia, A; Varalli, M; Santucci, R; Desideri, A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/49771
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