This paper describes an electrochemical biosensor for free cholesterol monitoring. The sensor is a multienzymatic electrodic system in which horseradish peroxidase and cholesterol oxidase are simultaneously immobilized within a polymeric film. on the surface of a pyrolitic graphite electrode. From voltammetric and amperometric (flow-injection) data obtained, the efficiency, reproducibility and stability of the system are discussed. Results obtained, of interest for basic and applied biochemistry, represent a first step for construction of a mediator-free biosensor with potentialities for a successful application in the biosensor area. (C) 2001 Elsevier Science BN. All rights reserved.
Bongiovanni, C., Ferri, T., Poscia, A., Varalli, M., Santucci, R., Desideri, A. (2001). An electrochemical multienzymatic biosensor for determination of cholesterol. BIOELECTROCHEMISTRY, 54(1), 17-22.
An electrochemical multienzymatic biosensor for determination of cholesterol
SANTUCCI, ROBERTO;DESIDERI, ALESSANDRO
2001-01-01
Abstract
This paper describes an electrochemical biosensor for free cholesterol monitoring. The sensor is a multienzymatic electrodic system in which horseradish peroxidase and cholesterol oxidase are simultaneously immobilized within a polymeric film. on the surface of a pyrolitic graphite electrode. From voltammetric and amperometric (flow-injection) data obtained, the efficiency, reproducibility and stability of the system are discussed. Results obtained, of interest for basic and applied biochemistry, represent a first step for construction of a mediator-free biosensor with potentialities for a successful application in the biosensor area. (C) 2001 Elsevier Science BN. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
