The assembly and the characterization (by DC cyclic voltammetry and flow injection analysis (FIA) mode) of a bienzymatic biosensor for glucose is described. The biosensor is constituted by a glucose oxidase (GOD)-entrapping cellulose triacetate membrane, layered onto a polymeric membrane (polystyrene cross-linked with 1% divinyl benzene functionalized with tributylmethyl phosphonium chloride (TBMPC)) embedding horseradish peroxidase (HRP), previously blocked onto a pyrolitic graphite electrode (PGE). Under flowing conditions, the biosensor, which gives place to direct, unmediated electron-transfer, responds linearly to the glucose up to 5.0 MM with a precison always better than 5%, and the detection limit was 0.07 mM. The influence of different experimental parameters, like temperature, flow rate, applied potential, interferents, etc., has been evaluated. For what concerns the potential interferences, no interfering species, as ascorbic acid, urea, etc., affects the biosensor response. The biosensor, characterized by an operational stability of at least twenty days, has been successfully tested on real samples.

Ferri, T., Maida, S., Poscia, A., Santucci, R. (2001). A glucose biosensor based on electro-enzyme catalyzed oxidation of glucose using a HRP-GOD layered assembly. ELECTROANALYSIS, 13(14), 1198-1202.

A glucose biosensor based on electro-enzyme catalyzed oxidation of glucose using a HRP-GOD layered assembly

SANTUCCI, ROBERTO
2001-01-01

Abstract

The assembly and the characterization (by DC cyclic voltammetry and flow injection analysis (FIA) mode) of a bienzymatic biosensor for glucose is described. The biosensor is constituted by a glucose oxidase (GOD)-entrapping cellulose triacetate membrane, layered onto a polymeric membrane (polystyrene cross-linked with 1% divinyl benzene functionalized with tributylmethyl phosphonium chloride (TBMPC)) embedding horseradish peroxidase (HRP), previously blocked onto a pyrolitic graphite electrode (PGE). Under flowing conditions, the biosensor, which gives place to direct, unmediated electron-transfer, responds linearly to the glucose up to 5.0 MM with a precison always better than 5%, and the detection limit was 0.07 mM. The influence of different experimental parameters, like temperature, flow rate, applied potential, interferents, etc., has been evaluated. For what concerns the potential interferences, no interfering species, as ascorbic acid, urea, etc., affects the biosensor response. The biosensor, characterized by an operational stability of at least twenty days, has been successfully tested on real samples.
2001
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
glucose biosensor; enzyme immobilization; horseradish peroxidase; layered assembly; biosensor
Ferri, T., Maida, S., Poscia, A., Santucci, R. (2001). A glucose biosensor based on electro-enzyme catalyzed oxidation of glucose using a HRP-GOD layered assembly. ELECTROANALYSIS, 13(14), 1198-1202.
Ferri, T; Maida, S; Poscia, A; Santucci, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/49769
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