The presence of hepatitis B virus (HBV) and human immunodeficiency virus (HIV) was investigated using hybridization in 15 lymph nodes and one Kaposi's sarcoma skin lesion obtained from HIV-positive patients. Cryostat tissue sections were hybridized with chemically modified DNA probes for HBV and HIV. HIV genome was mainly observed in the cytoplasm of cells present in 7 15 lymph nodes and in the Kaposi's sarcoma skin lesion, thus indicating the expression of HIV replication. Control samples hybridized with an HTLV I probe were negative. HBV genome was found in the cytoplasm of lymphoid mononuclear cells in 2 7 lymph nodes, obtained from HIV+ patients without serum markers of ongoing HBV infection. Lymph node positivity for HBV DNA also confirms that lymphoid cells may be a target for HBV. Since HBV infection seems to precede HIV infection in nearly all patients, it is possible that it may represent a factor facilitating the development of the HIV-related disease. Â© 1989.
Pezzella M., P.F. (1989). HBV and HIV expression in lymph nodes of HIV positive LAS patients: histology and in situ hybridization. MOLECULAR AND CELLULAR PROBES, 3(2), 125-132.
|Tipologia:||Articolo su rivista|
|Citazione:||Pezzella M., P.F. (1989). HBV and HIV expression in lymph nodes of HIV positive LAS patients: histology and in situ hybridization. MOLECULAR AND CELLULAR PROBES, 3(2), 125-132.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/14|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0890-8508(89)90023-6,|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||1989|
|Titolo:||HBV and HIV expression in lymph nodes of HIV positive LAS patients: histology and in situ hybridization|
|Autori:||Pezzella M., Pezzella F., Rapicetta M., Morace G., Macchi B., Sorice F., Baroni C.D.|
|Appare nelle tipologie:||01 - Articolo su rivista|