Objective: To study virologic and immunologic factors associated with discordant treatment response in HIV-infected patients receiving highly active antiretroviral therapy (HAART). Design: Study participants included a total of 27 patients: (a) 10 discordant patients (mean CD4+ cell count, 396.1 Ã— 106 cells/l; mean HIV-RNA, 5.4 log10 copies/ml); (b) seven responder patients (mean CD4+ cell count, 997.5 Ã— 106 cells/l); and (c) 10 failing patients (mean CD4+ cell count 66.5 Ã— 106 cells/l; mean HIV-RNA, 5.4 log10 copies/ml). Methods: The HIV-1 isolation rate and biological phenotype, drug resistance genotypic mutations of HIV-1 strains, recall and HIV-1-specific antigen lymphocyte proliferation (LP), and interleukin (IL)-15 production were studied. Results: Virus isolation was obtained in 30% of discordant patients, and in 100% of failing patients. A higher replication constant was reported in discordant patients. No difference in the number of drug resistance mutations and biological phenotypes of HIV-1 was found in discordant patients with respect to failing patients. Discordant patients developed positive LP responses to Candida albicans and HIV-1 p24. LP in response to C. albicans, HIV-1 p24 and gp160 was positive in responder patients. No significant LP was found in failing patients. Increased levels of IL-15 after stimulation with lipopolysaccaride (LPS) and C. albicans were found in both discordant patients and responder patients. Conversely, a strong reduction of IL-15 levels was observed in failing patients. Conclusion: The present results suggest that decreased virus isolation rate, restoration of both lymphocyte proliferation and IL-15 production are factors involved in the discordant antiretroviral therapy response of HIV-infected patients. Â© 2002 Lippincott Williams & Wilkins.
D'Ettorre, G., Forcina, G., Andreotti, M., Sarmati, L., Palmisano, L., Galluzzo, C.M., et al. (2002). Discordant response to antiretroviral therapy: HIV isolation, genotypic mutations, T-cell proliferation and cytokine production. AIDS, 16(14), 1877-1885.
|Tipologia:||Articolo su rivista|
|Citazione:||D'Ettorre, G., Forcina, G., Andreotti, M., Sarmati, L., Palmisano, L., Galluzzo, C.M., et al. (2002). Discordant response to antiretroviral therapy: HIV isolation, genotypic mutations, T-cell proliferation and cytokine production. AIDS, 16(14), 1877-1885.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore MED/17 - Malattie Infettive|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1097/00002030-200209270-00004|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2002|
|Titolo:||Discordant response to antiretroviral therapy: HIV isolation, genotypic mutations, T-cell proliferation and cytokine production|
|Autori:||D'Ettorre, G; Forcina, G; Andreotti, M; Sarmati, L; Palmisano, L; Galluzzo, CM; Nicastri, E; Mastroianni, CM; Vullo, V; Vella, S; Andreoni, M|
|Appare nelle tipologie:||01 - Articolo su rivista|