The aim of the study was to analyze the relationship between genotypic and phenotypic drug resistance profiles of human immunodeficiency virus type 1 (HIV-1) strains isolated from patients during double-analogue nucleoside therapy. A drug-resistant HIV strain was isolated from 20 out of 25 patients, with 16 (64%) subjects carrying a virus with multiple drug resistance mutations. The most frequent resistance mutations were M184V (18 isolates) and M41L (7 isolates). Discordance between the genotypic and phenotypic profile for at least one drug was detected in 16 out of 25 strains. Particularly, eight isolates had a discordant genotypic-phenotypic resistance pattern for two drugs and one isolate had such a pattern for three drugs. A genotypic resistance pattern with a phenotypic sensitivity profile was detected in six isolates (four resistant to zidovudine and two resistant to lamivudine). On the other hand for several strains a genotypic pattern of sensitivity pattern to abacavir (10 strains), didanosine (7 strains), stavudine (3 strains), zidovudine (2 strains), and lamivudine (1 strain) with a phenotypic resistance profile was detected. After a follow-up period of 8 months, an impairment of virological and immunological parameters was detected only in subjects with an HIV-1 isolate with a phenotypic resistance profile in despite of the genotypic results. Predicting resistance phenotype from genotypic data has important limitations. Despite the low number of patients and the short follow-up period, this study suggests that during failing therapy with analogue nucleosides, a phenotypic analysis could be performed in spite of an HIV genotypic sensitivity pattern.

Sarmati, L., Nicastri, E., Parisi, S., D'Ettorre, G., Mancino, G., Narciso, P., et al. (2002). Discordance between genotypic and phenotypic drug resistance profiles in human immunodeficiency virus type 1 strains isolated from peripheral blood mononuclear cells. JOURNAL OF CLINICAL MICROBIOLOGY, 40(2), 335-340 [10.1128/JCM.40.2.335-340.2002].

Discordance between genotypic and phenotypic drug resistance profiles in human immunodeficiency virus type 1 strains isolated from peripheral blood mononuclear cells

SARMATI, LOREDANA;ANDREONI, MASSIMO
2002-01-01

Abstract

The aim of the study was to analyze the relationship between genotypic and phenotypic drug resistance profiles of human immunodeficiency virus type 1 (HIV-1) strains isolated from patients during double-analogue nucleoside therapy. A drug-resistant HIV strain was isolated from 20 out of 25 patients, with 16 (64%) subjects carrying a virus with multiple drug resistance mutations. The most frequent resistance mutations were M184V (18 isolates) and M41L (7 isolates). Discordance between the genotypic and phenotypic profile for at least one drug was detected in 16 out of 25 strains. Particularly, eight isolates had a discordant genotypic-phenotypic resistance pattern for two drugs and one isolate had such a pattern for three drugs. A genotypic resistance pattern with a phenotypic sensitivity profile was detected in six isolates (four resistant to zidovudine and two resistant to lamivudine). On the other hand for several strains a genotypic pattern of sensitivity pattern to abacavir (10 strains), didanosine (7 strains), stavudine (3 strains), zidovudine (2 strains), and lamivudine (1 strain) with a phenotypic resistance profile was detected. After a follow-up period of 8 months, an impairment of virological and immunological parameters was detected only in subjects with an HIV-1 isolate with a phenotypic resistance profile in despite of the genotypic results. Predicting resistance phenotype from genotypic data has important limitations. Despite the low number of patients and the short follow-up period, this study suggests that during failing therapy with analogue nucleosides, a phenotypic analysis could be performed in spite of an HIV genotypic sensitivity pattern.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/17 - Malattie Infettive
English
Con Impact Factor ISI
abacavir; didanosine; lamivudine; stavudine; zidovudine; antibiotic sensitivity; article; clinical article; genotype; human; Human immunodeficiency virus 1; Human immunodeficiency virus infection; multidrug resistance; nonhuman; nucleotide sequence; phenotype; priority journal; virus mutation; virus resistance; virus strain; virus typing; Anti-HIV Agents; Drug Resistance, Viral; Drug Therapy, Combination; Genotype; HIV Infections; HIV-1; HIV-1 Reverse Transcriptase; Humans; Leukocytes, Mononuclear; Microbial Sensitivity Tests; Mutation; Phenotype; Reverse Transcriptase Inhibitors; Sequence Analysis, DNA; Human immunodeficiency virus; Human immunodeficiency virus 1; RNA viruses
Sarmati, L., Nicastri, E., Parisi, S., D'Ettorre, G., Mancino, G., Narciso, P., et al. (2002). Discordance between genotypic and phenotypic drug resistance profiles in human immunodeficiency virus type 1 strains isolated from peripheral blood mononuclear cells. JOURNAL OF CLINICAL MICROBIOLOGY, 40(2), 335-340 [10.1128/JCM.40.2.335-340.2002].
Sarmati, L; Nicastri, E; Parisi, S; D'Ettorre, G; Mancino, G; Narciso, P; Vullo, V; Andreoni, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/49472
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