The usefulness of traditional methods of HIV plasma titration has been limited by poor detection capacity in the asymptomatic phase of HIV disease. We analyzed plasma samples from asymptomatic seropositive or early symptomatic patients, comparing the classic plasma culture method with the following techniques: phorbol 12-myristate 13-acetate treatment of target cells, centrifugal inoculation of the virus, heat treatment of cultures, addition of monocyte/macrophages to cultures, and polyethylene glycol (PEG) treatment of the plasma. Only PEG treatment significantly increased the percentage of HIV isolation. The increase of HIV isolation after PEG treatment is more evident in patients with higher CD4+ cell counts and those without detectable levels of p24 antigen. In the p24-negative samples, HIV was isolated in 17 of 24 (71%) with PEG treatment versus nine of 24 (37%) with the classic method (p < 0.01). A number of discordant samples were found using the classic and PEG methods. Combining the positive results obtained with either technique, we obtained an overall HIV detection rate of 76%. The increased sensitivity of the combination of PEG and classic methods may allow a wider use of plasma viremia as part of the virological evaluation of anti- HIV drug efficacy in asymptomatic patients.

Sarmati, L., Ercoli, L., Parisi, S., Rocchi, G., Giannini, G., Galluzzo, C., et al. (1994). High rate of HIV isolation from plasma of asymptomatic patients through polyethylene glycol (PEG) treatment. JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, 7(1), 10-14.

High rate of HIV isolation from plasma of asymptomatic patients through polyethylene glycol (PEG) treatment

SARMATI, LOREDANA;ERCOLI, LUCIA;ANDREONI, MASSIMO
1994-01-01

Abstract

The usefulness of traditional methods of HIV plasma titration has been limited by poor detection capacity in the asymptomatic phase of HIV disease. We analyzed plasma samples from asymptomatic seropositive or early symptomatic patients, comparing the classic plasma culture method with the following techniques: phorbol 12-myristate 13-acetate treatment of target cells, centrifugal inoculation of the virus, heat treatment of cultures, addition of monocyte/macrophages to cultures, and polyethylene glycol (PEG) treatment of the plasma. Only PEG treatment significantly increased the percentage of HIV isolation. The increase of HIV isolation after PEG treatment is more evident in patients with higher CD4+ cell counts and those without detectable levels of p24 antigen. In the p24-negative samples, HIV was isolated in 17 of 24 (71%) with PEG treatment versus nine of 24 (37%) with the classic method (p < 0.01). A number of discordant samples were found using the classic and PEG methods. Combining the positive results obtained with either technique, we obtained an overall HIV detection rate of 76%. The increased sensitivity of the combination of PEG and classic methods may allow a wider use of plasma viremia as part of the virological evaluation of anti- HIV drug efficacy in asymptomatic patients.
1994
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/17 - MALATTIE INFETTIVE
English
Con Impact Factor ISI
antigen p24; macrogol; article; cell count; helper cell; human; human cell; human immunodeficiency virus; human immunodeficiency virus infection; nonhuman; priority journal; viremia; virus isolation; Cells, Cultured; Comparative Study; Heat; HIV; HIV Core Protein p24; HIV Seropositivity; Human; Macrophages; Monocytes; Polyethylene Glycols; Sensitivity and Specificity; Support, Non-U.S. Gov't; Tetradecanoylphorbol Acetate; Viremia
Sarmati, L., Ercoli, L., Parisi, S., Rocchi, G., Giannini, G., Galluzzo, C., et al. (1994). High rate of HIV isolation from plasma of asymptomatic patients through polyethylene glycol (PEG) treatment. JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, 7(1), 10-14.
Sarmati, L; Ercoli, L; Parisi, S; Rocchi, G; Giannini, G; Galluzzo, C; Vella, S; Andreoni, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/49467
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