Background: Recent studies have shown that nerve growth factor (NGF) is produced by and can act on several immune-inflammatory cells. Objectives: The objective of this study was to study the effects of NGF on human peripheral blood eosinophils and assess whether these cells produce and store NGF. Methods: Eosinophils were purified by negative immunoselection (magnetic cell sorting systems, purity 98% to 100%) from 13 subjects (9 to 26 years old) with mild blood eosinophilia, mainly of allergic origin. Eosinophils were incubated with NGF (50 to 1000 ng/mL), and supernatants were collected for measurement of eosinophil peroxidase (EPO, 20 minutes, colorimetric enzymatic assay) and IL-6 (12 hours, ELISA). Eosinophil viability was evaluated by Trypan blue test (days 2, 3, and 4). NGF content in freshly isolated eosinophils, after ultrasound disruption, was determined with a 2-site immunoenzymatic assay. The presence of mRNA for NGF was evaluated by reverse transcription PCR. Results: NGF caused EPO release (highly significant at 1000 ng/mL NGF). IL-6 release from eosinophils was not higher than IL-6 spontaneously released into culture medium alone. NGF did not significantly affect the number of viable eosinophils. NGF was found in the eosinophil sonicates (1.5 to 17.8 pg/mL per 106 cells). Similarly, mRNA for NGF was detected by reverse transcription PCR in the freshly isolated eosinophils. Conclusions: NGF activates human peripheral blood eosinophils from subjects with mild eosinophilia to selectively release inflammatory mediators. Eosinophils store and produce NGF. Therefore the capability of NGF to induce a secretory response and its production and storage by circulating human eosinophils suggest a possible role for NGF in conditions associated with eosinophilia, including allergic disease.
Solomon, A., Aloe, L., Pery, J., & Bonini S., L. (1998). Nerve growth factor is preformed in and activates human peripheral blood eosinophils. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 102(3), 454-460.
|Tipologia:||Articolo su rivista|
|Citazione:||Solomon, A., Aloe, L., Pery, J., & Bonini S., L. (1998). Nerve growth factor is preformed in and activates human peripheral blood eosinophils. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 102(3), 454-460.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore MED/15 - Malattie del Sangue|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/S0091-6749(98)70135-6|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||1998|
|Titolo:||Nerve growth factor is preformed in and activates human peripheral blood eosinophils|
|Autori:||Solomon, A.; Aloe, L.; Pery, J.F.; Bonini S., Levi-Schaffer, F.|
|Appare nelle tipologie:||01 - Articolo su rivista|