Background: The nosocomial infections surveillance system must be strongly effective especially in highly critic areas, such as Intensive Care Units (ICU). These areas are frequently an epidemiological epicentre for transmission of multi-resistant pathogens, like Acinetobacter baumannii. As an epidemic outbreak occurs it is very important to confirm or exclude the genetic relationship among the isolates in a short time. There are several molecular typing systems used with this aim. The Repetitive sequence-based PCR (REP-PCR) has been recognized as an effective method and it was recently adapted to an automated format known as the DiversiLab system. Methods: In the present study we have evaluated the combination of a newly introduced software package for the control of hospital infection (VIGI@ ct) with the DiversiLab system. In order to evaluate the reliability of the DiversiLab its results were also compared with those obtained using f-AFLP. Results: The combination of VIGI@ ct and DiversiLab enabled an earlier identification of an A. baumannii epidemic cluster, through the confirmation of the genetic relationship among the isolates. This cluster regards 56 multi-drug-resistant A. baumannii isolates from several specimens collected from 13 different patients admitted to the ICU in a ten month period. The A. baumannii isolates were clonally related being their similarity included between 97 and 100%. The results of the DiversiLab were confirmed by f-AFLP analysis. Conclusion: The early identification of the outbreak has led to the prompt application of operative procedures and precautions to avoid the spread of pathogen. To date, 6 months after the last A. baumannii isolate, no other related case has been identified.

Fontana, C., Favaro, M., Minelli, S., Bossa, M., Testore, G., Leonardis, F., et al. (2008). Acinetobacter baumannii in intensive care unit: A novel system to study clonal relationship among the isolates. BMC INFECTIOUS DISEASES, 8, 79 [10.1186/1471-2334-8-79].

Acinetobacter baumannii in intensive care unit: A novel system to study clonal relationship among the isolates

FONTANA, CARLA;FAVARO, MARCO;LEONARDIS, FRANCESCA;NATOLI, SILVIA;FAVALLI, CARTESIO
2008-01-01

Abstract

Background: The nosocomial infections surveillance system must be strongly effective especially in highly critic areas, such as Intensive Care Units (ICU). These areas are frequently an epidemiological epicentre for transmission of multi-resistant pathogens, like Acinetobacter baumannii. As an epidemic outbreak occurs it is very important to confirm or exclude the genetic relationship among the isolates in a short time. There are several molecular typing systems used with this aim. The Repetitive sequence-based PCR (REP-PCR) has been recognized as an effective method and it was recently adapted to an automated format known as the DiversiLab system. Methods: In the present study we have evaluated the combination of a newly introduced software package for the control of hospital infection (VIGI@ ct) with the DiversiLab system. In order to evaluate the reliability of the DiversiLab its results were also compared with those obtained using f-AFLP. Results: The combination of VIGI@ ct and DiversiLab enabled an earlier identification of an A. baumannii epidemic cluster, through the confirmation of the genetic relationship among the isolates. This cluster regards 56 multi-drug-resistant A. baumannii isolates from several specimens collected from 13 different patients admitted to the ICU in a ten month period. The A. baumannii isolates were clonally related being their similarity included between 97 and 100%. The results of the DiversiLab were confirmed by f-AFLP analysis. Conclusion: The early identification of the outbreak has led to the prompt application of operative procedures and precautions to avoid the spread of pathogen. To date, 6 months after the last A. baumannii isolate, no other related case has been identified.
2008
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/41 - ANESTESIOLOGIA
English
Con Impact Factor ISI
amikacin; ampicillin; aztreonam; carbapenem; cephalosporin; ciprofloxacin; colistin; gentamicin; imipenem; meropenem; piperacillin plus tazobactam; Acinetobacter baumannii; adult; amplified fragment length polymorphism; antibiotic sensitivity; article; bacterium culture; bacterium identification; bacterium isolate; Candida albicans; clinical article; data analysis software; Enterococcus faecalis; Enterococcus faecium; Escherichia coli; female; gene cluster; genetic similarity; hospital infection; human; infection control; intensive care unit; intermethod comparison; Klebsiella pneumoniae; male; molecular phylogeny; molecular typing; multidrug resistance; nonhuman; Pseudomonas aeruginosa; Staphylococcus aureus; Staphylococcus epidermidis; Staphylococcus haemolyticus; bacterial infection; cell clone; classification; computer program; cross infection; disease transmission; DNA fingerprinting; epidemic; evaluation; genetics; health survey; hospital information system; instrumentation; isolation and purification; Italy; methodology; microbiology; middle aged; polymerase chain reaction; Acinetobacter baumannii; Acinetobacter Infections; Amplified Fragment Length Polymorphism Analysis; Clinical Laboratory Information Systems; Clone Cells; Cross Infection; Disease Outbreaks; DNA Fingerprinting; Environmental Microbiology; Female; Humans; Infection Control; Intensive Care Units; Italy; Male; Middle Aged; Polymerase Chain Reaction; Sentinel Surveillance; Software
Fontana, C., Favaro, M., Minelli, S., Bossa, M., Testore, G., Leonardis, F., et al. (2008). Acinetobacter baumannii in intensive care unit: A novel system to study clonal relationship among the isolates. BMC INFECTIOUS DISEASES, 8, 79 [10.1186/1471-2334-8-79].
Fontana, C; Favaro, M; Minelli, S; Bossa, M; Testore, G; Leonardis, F; Natoli, S; Favalli, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/49271
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