Growing evidence indicates that viral replication is regulated by the redox state of the host cell. We demonstrate that cells of different origins display differential permissivity for influenza A virus replication, depending on their intracellular redox power as reflected by Bcl-2 expression and glutathione (GSH) content. Bcl-2 expressing cells were found to have higher intracellular levels of GSH and to produce lower amounts of virus than Bcl-2 negative cells. Two different steps in the virus life-cycle were involved in Bcl-2/GSH mediated viral inhibition: 1) expression of late viral proteins (in particular hemagglutinin and matrix); and 2) nuclear-cytoplasmic translocation of viral ribonucleoproteins (vRNPs). Buthionine-sulfoximine-induced inhibition of GSH synthesis in Bcl-2 expressing cells caused an increase in the expression of late viral proteins but did not restore vRNP export to the cytoplasm. Collectively, our findings show that both Bcl-2 expression and GSH content contribute to the host cell's ability to down-regulate influenza virus replication, although their effects are exerted at different stages of the viral life-cycle. In certain cell populations, this form of down-regulation might conceivably favor the establishment of persistent viral infection.
Nencioni, L., Iuvara, A., Aquilano, K., Ciriolo, M.R., Cozzolino, F., Rotilio, G., et al. (2003). Influenza A virus replication is dependent on an antioxidant pathway that involves GSH and Bcl-2. THE FASEB JOURNAL, 17(6), 758-760 [10.1096/fj.02-0508fje].
|Tipologia:||Articolo su rivista|
|Citazione:||Nencioni, L., Iuvara, A., Aquilano, K., Ciriolo, M.R., Cozzolino, F., Rotilio, G., et al. (2003). Influenza A virus replication is dependent on an antioxidant pathway that involves GSH and Bcl-2. THE FASEB JOURNAL, 17(6), 758-760 [10.1096/fj.02-0508fje].|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/10|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1096/fj.02-0508fje|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2003|
|Titolo:||Influenza A virus replication is dependent on an antioxidant pathway that involves GSH and Bcl-2|
|Autori:||Nencioni, L; Iuvara, A; Aquilano, K; Ciriolo, MR; Cozzolino, F; Rotilio, G; Garaci, E; Palamara, AT|
|Appare nelle tipologie:||01 - Articolo su rivista|