Autoantibodies against U3 small nuclear ribonucleoprotein are associated with scleroderma autoimmune disease. They were shown to react with fibrillarin, a 34- to 36-kilodalton protein that has been detected in all eukaryotes tested from humans to yeasts. We isolated a 1.6-kilobase cDNA encoding fibrillarin from a Xenopus laevis cDNA library. The protein contains a 79-residue-long Gly-Arg-rich domain in its N-terminal region and a putative RNA-binding domain with ribonucleoprotein consensus sequence in its central portion. This is the first report of cloning of fibrillarin, and the deduced protein sequence is in agreement with the involvement of the protein in a ribonucleoprotein particle.
Lapeyre, B., Mariottini, P., Mathieu, C., Ferrer, P., Amaldi, F., Amalric, F., et al. (1990). Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease. MOLECULAR AND CELLULAR BIOLOGY, 10(1), 430-434 [10.1128/MCB.10.1.430].
Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease
AMALDI, FRANCESCO;
1990-01-01
Abstract
Autoantibodies against U3 small nuclear ribonucleoprotein are associated with scleroderma autoimmune disease. They were shown to react with fibrillarin, a 34- to 36-kilodalton protein that has been detected in all eukaryotes tested from humans to yeasts. We isolated a 1.6-kilobase cDNA encoding fibrillarin from a Xenopus laevis cDNA library. The protein contains a 79-residue-long Gly-Arg-rich domain in its N-terminal region and a putative RNA-binding domain with ribonucleoprotein consensus sequence in its central portion. This is the first report of cloning of fibrillarin, and the deduced protein sequence is in agreement with the involvement of the protein in a ribonucleoprotein particle.| Campo DC | Valore | Lingua |
|---|---|---|
| dc.authority.academicField2000 | Settore BIO/11 - BIOLOGIA MOLECOLARE | it |
| dc.authority.ancejournal | MOLECULAR AND CELLULAR BIOLOGY | - |
| dc.authority.isicrui | Biologia Molecolare e Genetica | it |
| dc.authority.people | Lapeyre, B | - |
| dc.authority.people | Mariottini, P | - |
| dc.authority.people | Mathieu, C | - |
| dc.authority.people | Ferrer, P | - |
| dc.authority.people | AMALDI, FRANCESCO | it |
| dc.authority.people | Amalric, F | - |
| dc.authority.people | Caizergues Ferrer, M. | - |
| dc.cilea.antefix | yes | it |
| dc.collection.id.s | e291c0df-b2a9-cddb-e053-3a05fe0aa144 | * |
| dc.collection.name | 01 - Articolo su rivista | * |
| dc.contributor.appartenenza | Dipartimento di Biologia | * |
| dc.contributor.appartenenza.mi | 7078 | * |
| dc.contributor.area | Non assegnato | * |
| dc.date.accessioned | 2012-03-08T12:32:33Z | - |
| dc.date.available | 2012-03-08T12:32:33Z | - |
| dc.date.created | 1990 | - |
| dc.date.issued | 1990 | - |
| dc.description.abstracteng | Autoantibodies against U3 small nuclear ribonucleoprotein are associated with scleroderma autoimmune disease. They were shown to react with fibrillarin, a 34- to 36-kilodalton protein that has been detected in all eukaryotes tested from humans to yeasts. We isolated a 1.6-kilobase cDNA encoding fibrillarin from a Xenopus laevis cDNA library. The protein contains a 79-residue-long Gly-Arg-rich domain in its N-terminal region and a putative RNA-binding domain with ribonucleoprotein consensus sequence in its central portion. This is the first report of cloning of fibrillarin, and the deduced protein sequence is in agreement with the involvement of the protein in a ribonucleoprotein particle. | - |
| dc.description.allpeople | Lapeyre, B; Mariottini, P; Mathieu, C; Ferrer, P; Amaldi, F; Amalric, F; Caizergues Ferrer, M | - |
| dc.description.allpeopleoriginal | Lapeyre, B; Mariottini, P; Mathieu, C; Ferrer, P; Amaldi, F; Amalric, F; Caizergues-Ferrer, M | it |
| dc.description.fulltext | none | en |
| dc.description.numberofauthors | 7 | - |
| dc.identifier.citation | Lapeyre, B., Mariottini, P., Mathieu, C., Ferrer, P., Amaldi, F., Amalric, F., et al. (1990). Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease. MOLECULAR AND CELLULAR BIOLOGY, 10(1), 430-434 [10.1128/MCB.10.1.430]. | it |
| dc.identifier.doi | 10.1128/MCB.10.1.430 | it |
| dc.identifier.uri | http://hdl.handle.net/2108/48181 | - |
| dc.language.iso | eng | it |
| dc.publisher.name | American Society for Microbiology | it |
| dc.relation.firstpage | 430 | it |
| dc.relation.issue | 1 | it |
| dc.relation.lastpage | 434 | it |
| dc.relation.volume | 10 | it |
| dc.subject.keywordseng | Animals; Autoimmune Diseases; Blotting, Northern; Recombinant Proteins; Ribonucleoproteins, Small Nuclear; RNA, Small Nuclear; Amino Acid Sequence; Autoantigens; Binding Sites; Cloning, Molecular; Chromosomal Proteins, Non-Histone; Xenopus laevis; Base Sequence; Molecular Sequence Data; Ribonucleoproteins | - |
| dc.subject.singlekeyword | Animals | * |
| dc.subject.singlekeyword | Autoimmune Diseases | * |
| dc.subject.singlekeyword | Blotting | * |
| dc.subject.singlekeyword | Northern | * |
| dc.subject.singlekeyword | Recombinant Proteins | * |
| dc.subject.singlekeyword | Ribonucleoproteins | * |
| dc.subject.singlekeyword | Small Nuclear | * |
| dc.subject.singlekeyword | RNA | * |
| dc.subject.singlekeyword | Small Nuclear | * |
| dc.subject.singlekeyword | Amino Acid Sequence | * |
| dc.subject.singlekeyword | Autoantigens | * |
| dc.subject.singlekeyword | Binding Sites | * |
| dc.subject.singlekeyword | Cloning | * |
| dc.subject.singlekeyword | Molecular | * |
| dc.subject.singlekeyword | Chromosomal Proteins | * |
| dc.subject.singlekeyword | Non-Histone | * |
| dc.subject.singlekeyword | Xenopus laevis | * |
| dc.subject.singlekeyword | Base Sequence | * |
| dc.subject.singlekeyword | Molecular Sequence Data | * |
| dc.subject.singlekeyword | Ribonucleoproteins | * |
| dc.title | Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease | it |
| dc.type | Articolo su rivista | - |
| dc.type.circulation | Rilevanza internazionale | it |
| dc.type.contribution | Articolo | it |
| dc.type.driver | info:eu-repo/semantics/article | - |
| dc.type.full | Pubblicazioni::01 - Articolo su rivista | it |
| dc.type.impactfactor | si | it |
| dc.type.miur | 262 | en |
| dc.type.publicationstatus | Pubblicato | it |
| dc.type.referee | Sì, ma tipo non specificato | it |
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| Appare nelle tipologie: | 01 - Articolo su rivista | |
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