We investigated the presence and localization, in the cells of anucleolate mutant embryos of Xenopus laevis, of three representative small nucleolar RNAs (snoRNAs), U3, U15 and U17, and of two nucleolar proteins, nucleolin and fibrillarin. The levels of the three snoRNAs in the anucleolate mutant are the same as in normal embryos, in contrast to 5S RNA and ribosomal proteins. In situ hybridization showed that, in the absence of fully organized nucleoli, the three RNAs are diffusely distributed in the nucleus and partly associated with a number of small structures. Nucleolin and fibrillarin are also present in the anucleolate embryos as in normal embryos, although there is less nucleolin mRNA in the former. The two nucleolar proteins were localized by immunofluorescence microscopy. Fibrillarin, similar to its associated U3 and U15 snoRNAs, is diffusely distributed in the anucleolate nucleus and is partly associated with small structures, probably prenucleolar bodies and pseudonucleoli. Nucleolin also appears diffusely distributed in the nucleus with some spots of higher concentration, but with a different pattern with respect to fibrillarin.

Crosio, C., Campioni, N., Cardinali, B., Amaldi, F., & Pierandrei-Amaldi, P. (1997). Small nucleolar RNAs and nucleolar proteins in xenopus anucleolate embryos. CHROMOSOMA, 105, 452-458 [10.1007/s004120050207].

Small nucleolar RNAs and nucleolar proteins in xenopus anucleolate embryos

AMALDI, FRANCESCO;
1997

Abstract

We investigated the presence and localization, in the cells of anucleolate mutant embryos of Xenopus laevis, of three representative small nucleolar RNAs (snoRNAs), U3, U15 and U17, and of two nucleolar proteins, nucleolin and fibrillarin. The levels of the three snoRNAs in the anucleolate mutant are the same as in normal embryos, in contrast to 5S RNA and ribosomal proteins. In situ hybridization showed that, in the absence of fully organized nucleoli, the three RNAs are diffusely distributed in the nucleus and partly associated with a number of small structures. Nucleolin and fibrillarin are also present in the anucleolate embryos as in normal embryos, although there is less nucleolin mRNA in the former. The two nucleolar proteins were localized by immunofluorescence microscopy. Fibrillarin, similar to its associated U3 and U15 snoRNAs, is diffusely distributed in the anucleolate nucleus and is partly associated with small structures, probably prenucleolar bodies and pseudonucleoli. Nucleolin also appears diffusely distributed in the nucleus with some spots of higher concentration, but with a different pattern with respect to fibrillarin.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/11
eng
Con Impact Factor ISI
18S RIBOSOMAL-RNA; U3 SNRNA; LAEVIS; FIBRILLARIN; EXPRESSION; SEQUENCE; GENE; CLONING; INTRONS
Crosio, C., Campioni, N., Cardinali, B., Amaldi, F., & Pierandrei-Amaldi, P. (1997). Small nucleolar RNAs and nucleolar proteins in xenopus anucleolate embryos. CHROMOSOMA, 105, 452-458 [10.1007/s004120050207].
Crosio, C; Campioni, N; Cardinali, B; Amaldi, F; Pierandrei Amaldi, P
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/48136
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