Vascular endothelial growth factor receptor-1 (VEGFR-1) exists in two isoforms: a membrane-bound isoform (mVEGFR-1) and a soluble one (sVEGFR-1). mVEGFR-1 is involved in endothelial cell migration and survival supported by VEGF-A and placenta growth factor (PIGF), whereas the biologic function of sVEGFR-1 has not been fully elucidated. We previously reported that sVEGFR-1 induces endothelial cell motility and promotes endothelial cell adhesion. In this study, we tested a set of VEGFR-1-derived peptides for their ability to interfere with endothelial cell migration. Peptide B3 was found to specifically inhibit cell migration induced by sVEGFR-1 and by mVEGFR-1-specific ligands. Moreover, peptide B3 markedly hampered angiogenesis in vitro and in vivo and was found to interfere with VEGFR-1 homodimerisation. Altogether, these data demonstrate that peptide B3 might be a useful tool for the specific inhibition of VEGFR-1 function and might represent a basis for the development of new anti-angiogenic compounds. (C) 2008 Elsevier Ltd. All rights reserved.

Lacal, P.m., Morea, V., Ruffini, F., Orecchia, A., Dorio, A.s., Failla, C.m., et al. (2008). Inhibition of endothelial cell migration and angiogenesis by a vascular endothelial growth factor receptor-1 derived peptide. EUROPEAN JOURNAL OF CANCER, 44(13), 1914-1921 [10.1016/j.ejca.2008.06.032].

Inhibition of endothelial cell migration and angiogenesis by a vascular endothelial growth factor receptor-1 derived peptide

DORIO, ANNALISA SUSANNA;TENTORI, LUCIO;GRAZIANI, GRAZIA;
2008-09

Abstract

Vascular endothelial growth factor receptor-1 (VEGFR-1) exists in two isoforms: a membrane-bound isoform (mVEGFR-1) and a soluble one (sVEGFR-1). mVEGFR-1 is involved in endothelial cell migration and survival supported by VEGF-A and placenta growth factor (PIGF), whereas the biologic function of sVEGFR-1 has not been fully elucidated. We previously reported that sVEGFR-1 induces endothelial cell motility and promotes endothelial cell adhesion. In this study, we tested a set of VEGFR-1-derived peptides for their ability to interfere with endothelial cell migration. Peptide B3 was found to specifically inhibit cell migration induced by sVEGFR-1 and by mVEGFR-1-specific ligands. Moreover, peptide B3 markedly hampered angiogenesis in vitro and in vivo and was found to interfere with VEGFR-1 homodimerisation. Altogether, these data demonstrate that peptide B3 might be a useful tool for the specific inhibition of VEGFR-1 function and might represent a basis for the development of new anti-angiogenic compounds. (C) 2008 Elsevier Ltd. All rights reserved.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/14
English
Con Impact Factor ISI
Angiogenesis; Endothelial cells; Migration; PlGF; Soluble VEGFR-1; VEGFR-1 activation; VEGFR-1 peptides
Lacal, P.m., Morea, V., Ruffini, F., Orecchia, A., Dorio, A.s., Failla, C.m., et al. (2008). Inhibition of endothelial cell migration and angiogenesis by a vascular endothelial growth factor receptor-1 derived peptide. EUROPEAN JOURNAL OF CANCER, 44(13), 1914-1921 [10.1016/j.ejca.2008.06.032].
Lacal, Pm; Morea, V; Ruffini, F; Orecchia, A; Dorio, As; Failla, Cm; Soro, S; Tentori, L; Zambruno, G; Graziani, G; Tramontano, A; D'Atri, S
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/47532
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