The present research was aimed to prevent mother-to-child transmission of HIV; to use RT-PCR in order to detect, 6 months after birth, infected children; and to test the antiretroviral resistance of both children and mothers in order to offer them a suitable therapy. At the Saint Camille Medical Centre, 3,127 pregnant women (aged 15-44 years) accepted to be enrolled in the mother-to-child transmission prevention protocol that envisages: (i) Voluntary Counselling and Testing for all the pregnant women; (ii) Antiretroviral therapy for HIV positive pregnant women and for their newborns; (iii) either powdered milk feeding or short breast-feeding and RT-PCR test for their children; (iv) finally, pol gene sequencing and antiretroviral resistance identifications among HIV positive mothers and children. Among the patients, 227/3,127 HIV seropositive women were found: 221/227 HIV-1, 4/227 HIV-2, and 2/227 mixed HIV infections. The RT-PCR test allowed the detection of 3/213 (1.4%) HIV infected children: 0/109 (0%) from mothers under ARV therapy and 3/104 (2.8%) from mothers treated with Nevirapine. All children had recombinant HIV-1 strain (CRF06_CPX) with: minor PR mutations (M36I, K20I) and RT mutations (R211K). Among them, two twins had Non-Nucleoside Reverse Transcriptase Inhibitor mutation (Y18CY). Both mothers acquired a major PR mutation (V8IV), investigated 6 months after a single-dose of Nevirapine. Prevention by single-dose of Nevirapine reduced significantly mother-to-child transmission of HIV, but caused many mutations and resistance to antiretroviral drugs. Based on present study the antiretroviral therapy protocol, together with the artificial-feeding, might represent the ideal strategy to avoid transmission of HIV from mother-to-child.

Simpore, J., Pietra, V., Pignatelli, S., Karou, D., Nadembega, W., Ilboudo, D., et al. (2007). Effective program against mother-to-child transmission of HIV at Saint Camille Medical Centre in Burkina Faso. JOURNAL OF MEDICAL VIROLOGY, 79(7), 873-879 [10.1002/jmv.20913].

Effective program against mother-to-child transmission of HIV at Saint Camille Medical Centre in Burkina Faso

CECCHERINI SILBERSTEIN, FRANCESCA;Bellocchi, M;COLIZZI, VITTORIO;PERNO, CARLO FEDERICO;
2007-07-01

Abstract

The present research was aimed to prevent mother-to-child transmission of HIV; to use RT-PCR in order to detect, 6 months after birth, infected children; and to test the antiretroviral resistance of both children and mothers in order to offer them a suitable therapy. At the Saint Camille Medical Centre, 3,127 pregnant women (aged 15-44 years) accepted to be enrolled in the mother-to-child transmission prevention protocol that envisages: (i) Voluntary Counselling and Testing for all the pregnant women; (ii) Antiretroviral therapy for HIV positive pregnant women and for their newborns; (iii) either powdered milk feeding or short breast-feeding and RT-PCR test for their children; (iv) finally, pol gene sequencing and antiretroviral resistance identifications among HIV positive mothers and children. Among the patients, 227/3,127 HIV seropositive women were found: 221/227 HIV-1, 4/227 HIV-2, and 2/227 mixed HIV infections. The RT-PCR test allowed the detection of 3/213 (1.4%) HIV infected children: 0/109 (0%) from mothers under ARV therapy and 3/104 (2.8%) from mothers treated with Nevirapine. All children had recombinant HIV-1 strain (CRF06_CPX) with: minor PR mutations (M36I, K20I) and RT mutations (R211K). Among them, two twins had Non-Nucleoside Reverse Transcriptase Inhibitor mutation (Y18CY). Both mothers acquired a major PR mutation (V8IV), investigated 6 months after a single-dose of Nevirapine. Prevention by single-dose of Nevirapine reduced significantly mother-to-child transmission of HIV, but caused many mutations and resistance to antiretroviral drugs. Based on present study the antiretroviral therapy protocol, together with the artificial-feeding, might represent the ideal strategy to avoid transmission of HIV from mother-to-child.
lug-2007
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
English
Con Impact Factor ISI
Infectious Disease Transmission, Vertical; Anti-HIV Agents; Burkina Faso; Humans; Pregnancy Complications, Infectious; Infant, Newborn; Reverse Transcriptase Polymerase Chain Reaction; HIV-1; HIV-2; Pregnancy; Infant; Drug Resistance, Viral; HIV Infections; Adult; Adolescent; Mutation; Male; Female
Simpore, J., Pietra, V., Pignatelli, S., Karou, D., Nadembega, W., Ilboudo, D., et al. (2007). Effective program against mother-to-child transmission of HIV at Saint Camille Medical Centre in Burkina Faso. JOURNAL OF MEDICAL VIROLOGY, 79(7), 873-879 [10.1002/jmv.20913].
Simpore, J; Pietra, V; Pignatelli, S; Karou, D; Nadembega, W; Ilboudo, D; CECCHERINI SILBERSTEIN, F; Ghilat Avoid Belem, W; Bellocchi, M; Saleri, N; S...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/45967
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