Reactive oxygen species (ROS) and redox state have emerged as physiological mediators, controlling blood coagulation and thrombosis. The redox balance is obviously linked to the presence of antioxidants; in particular, vitamin C appears to be a key modulator of platelet oxidative state, since these cells physiologically accumulate ascorbic acid and, moreover, platelet ascorbate plays a role during aggregation. Here, we showed that platelets could compensate for fluctuations in ascorbate levels by modulating the expression of the Na+-dependent transporter SVCT2. Furthermore, the use of anucleated cells demonstrated, for the first time, that SVCT2 expression could be regulated at the translational level. The control of ascorbic acid uptake, through regulation of its carrier, was not only related to substrate availability, but it also occurred during platelet activation, which was accompanied by vitamin C deprivation and alteration in the redox state. Finally, we showed that changes in intracellular ascorbic acid content had physiological relevance, since they modulate the surface sulfhydryl content and the thrombus viscoelastic properties. Beside its role during aggregation, vitamin C may also have important effects during postaggregatory events. (c) 2006 Elsevier Inc. All rights reserved.
Savini, I., Catani, M.V., Arnone, R., Rossi, A., Frega, G., Del Principe, D., et al. (2007). Translational control of the ascorbic acid transporter SVCT2 in human platelets. FREE RADICAL BIOLOGY & MEDICINE, 42(5), 608-616.
|Tipologia:||Articolo su rivista|
|Citazione:||Savini, I., Catani, M.V., Arnone, R., Rossi, A., Frega, G., Del Principe, D., et al. (2007). Translational control of the ascorbic acid transporter SVCT2 in human platelets. FREE RADICAL BIOLOGY & MEDICINE, 42(5), 608-616.|
|Altre informazioni significative:||9|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore MED/49 - Scienze Tecniche Dietetiche Applicate|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.freeradbiomed.2006.11.028|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2007|
|Titolo:||Translational control of the ascorbic acid transporter SVCT2 in human platelets|
|Autori:||Savini, I; Catani, MV; Arnone, R; Rossi, A; Frega, G; Del Principe, D; Avigliano, L|
|Appare nelle tipologie:||01 - Articolo su rivista|