The substrate binding mechanism and catalysis of the human glutathione transferase T2-2 have been studied and compared to those found in the more recently evolved Alpha, Mu and Pi class isoenzymes. The evolution strategy for the optimization of the binding process is based on the shift from a single-step to a multi-step interaction with the substrate. An evolutionary pressure in terms of flexibility of the protein and of proper orientation of the substrate may also explain the relevant differences of the catalytic properties found among the 'old' and 'young' isoenzymes. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
Caccuri, A.M., Antonini, G., Board, P.G., Flanagan, J., Parker, M.W., Paolesse, R., et al. (2001). Evolutionary strategy for optimization of GSH binding and catalysis in Alpha, Pi, Mu and theta glutathione transferases. CHEMICO-BIOLOGICAL INTERACTIONS, 133, 163-166.
Tipologia: | Articolo su rivista |
Citazione: | Caccuri, A.M., Antonini, G., Board, P.G., Flanagan, J., Parker, M.W., Paolesse, R., et al. (2001). Evolutionary strategy for optimization of GSH binding and catalysis in Alpha, Pi, Mu and theta glutathione transferases. CHEMICO-BIOLOGICAL INTERACTIONS, 133, 163-166. |
IF: | Con Impact Factor ISI |
Lingua: | English |
Settore Scientifico Disciplinare: | Settore BIO/10 Settore CHIM/07 - Fondamenti Chimici delle Tecnologie |
Revisione (peer review): | Sì, ma tipo non specificato |
Tipo: | Articolo |
Rilevanza: | Rilevanza internazionale |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1074/jbc.M002819200 |
Stato di pubblicazione: | Pubblicato |
Data di pubblicazione: | 2001 |
Titolo: | Evolutionary strategy for optimization of GSH binding and catalysis in Alpha, Pi, Mu and theta glutathione transferases |
Autori: | |
Autori: | Caccuri, AM; Antonini, G; Board, PG; Flanagan, J; Parker, MW; Paolesse, R; Turella, P; Federici, G; Lo Bello, M; Ricci, G |
Appare nelle tipologie: | 01 - Articolo su rivista |