We have identified an N-capping box motif (Ser/Thr-Xaa-Xaa-Asp) that is strictly conserved, at the beginning of alpha 6 helix, in all glutathione S-transferases (GSTs) and most of the related superfamily proteins, By using CD and peptide modelling we demonstrated that the capping box residues have an important role in determining the helical conformation adopted by this fragment in the hydrophobic environment of the protein. This is an example in which a local motif, contributing to nucleation of a structural element essential to the global folding of the protein, is strictly conserved in a superfamily of homologous proteins.

Aceto, A., Dragani, B., Melino, S.m., Allocati, N., Masulli, M., Di Ilio, C., et al. (1997). Identification of an N-capping box that affects the alpha 6-helix propensity in glutathione S-transferase superfamily proteins: A role for an invariant aspartic residue. BIOCHEMICAL JOURNAL, 322(1), 229-234.

Identification of an N-capping box that affects the alpha 6-helix propensity in glutathione S-transferase superfamily proteins: A role for an invariant aspartic residue

MELINO, SONIA MICHAELA;
1997-01-01

Abstract

We have identified an N-capping box motif (Ser/Thr-Xaa-Xaa-Asp) that is strictly conserved, at the beginning of alpha 6 helix, in all glutathione S-transferases (GSTs) and most of the related superfamily proteins, By using CD and peptide modelling we demonstrated that the capping box residues have an important role in determining the helical conformation adopted by this fragment in the hydrophobic environment of the protein. This is an example in which a local motif, contributing to nucleation of a structural element essential to the global folding of the protein, is strictly conserved in a superfamily of homologous proteins.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10
English
Con Impact Factor ISI
SITE-DIRECTED MUTAGENESIS; 3-DIMENSIONAL STRUCTURE; HELIX STOP; GENE; RESOLUTION; ELONGATION; EXPRESSION; PEPTIDES; COMPLEX; SIGNALS
Aceto, A., Dragani, B., Melino, S.m., Allocati, N., Masulli, M., Di Ilio, C., et al. (1997). Identification of an N-capping box that affects the alpha 6-helix propensity in glutathione S-transferase superfamily proteins: A role for an invariant aspartic residue. BIOCHEMICAL JOURNAL, 322(1), 229-234.
Aceto, A; Dragani, B; Melino, Sm; Allocati, N; Masulli, M; Di Ilio, C; Petruzzelli, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/44455
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