Bub1 is a component of the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures genome stability by delaying anaphase until all the chromosomes are stably attached to spindle microtubules via their kinetochores. To define Bub1's role in chromosome segregation, embryogenesis, and tissue homeostasis, we generated a mouse strain in which BUB1 can be inactivated by administration of tamoxifen, thereby bypassing the preimplantation lethality associated with the Bub1 null phenotype. We show that Bub1 is essential for postimplantation embryogenesis and proliferation of primary embryonic fibroblasts. Bub1 inactivation in adult males inhibits proliferation in seminiferous tubules, reducing sperm production and causing infertility. In culture, Bub1-deficient fibroblasts fail to align their chromosomes or sustain SAC function, yielding a highly aberrant mitosis that prevents further cell divisions. Centromeres in Bub1-deficient cells also separate prematurely; however, we show that this is a consequence of SAC dysfunction rather than a direct role for Bub1 in protecting centromeric cohesion.

Perera, D., Tilston, V., Hopwood, J., Barchi, M., Boot Handford, R., & Taylor, S. (2007). Bub1 maintains centromeric cohesion by activation of the spindle checkpoint. DEVELOPMENTAL CELL, 566-579 [10.1016/j.devcel.2007.08.008].

Bub1 maintains centromeric cohesion by activation of the spindle checkpoint.

BARCHI, MARCO;
2007-10-09

Abstract

Bub1 is a component of the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures genome stability by delaying anaphase until all the chromosomes are stably attached to spindle microtubules via their kinetochores. To define Bub1's role in chromosome segregation, embryogenesis, and tissue homeostasis, we generated a mouse strain in which BUB1 can be inactivated by administration of tamoxifen, thereby bypassing the preimplantation lethality associated with the Bub1 null phenotype. We show that Bub1 is essential for postimplantation embryogenesis and proliferation of primary embryonic fibroblasts. Bub1 inactivation in adult males inhibits proliferation in seminiferous tubules, reducing sperm production and causing infertility. In culture, Bub1-deficient fibroblasts fail to align their chromosomes or sustain SAC function, yielding a highly aberrant mitosis that prevents further cell divisions. Centromeres in Bub1-deficient cells also separate prematurely; however, we show that this is a consequence of SAC dysfunction rather than a direct role for Bub1 in protecting centromeric cohesion.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/16
English
Con Impact Factor ISI
Perera, D., Tilston, V., Hopwood, J., Barchi, M., Boot Handford, R., & Taylor, S. (2007). Bub1 maintains centromeric cohesion by activation of the spindle checkpoint. DEVELOPMENTAL CELL, 566-579 [10.1016/j.devcel.2007.08.008].
Perera, D; Tilston, V; Hopwood, J; Barchi, M; Boot Handford, R; Taylor, S
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/44189
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