We have analyzed several randomly selected mRNAs, of the relatively abundant category, on the basis of maternal or zygotic origin and translational efficiency at different developmental stages. For this purpose, clones from a Xenopus embryo cDNA library were hybridized with cDNA probes prepared with poly(A) + RNA from polysomes and from mRNPs of embryos at different stages. The results obtained indicate that the majority of the relatively abundant MRNAs (38 out of 61) is subject to some kind of translational regulation during embryogenesis. Moreover, 30 clones have been selected as corresponding to mRNAs that behave, from the point of view of transcriptional and translational regulation, similarly to previously studied ribosomal protein (r-protein) mRNAs. Sequence analysis of 20 of these selected cDNAs has shown that half of them are in fact homologous to already sequenced r-protein mRNAs. Unexpectedly we have found that also the mRNA for alpha-cardiac actin and another mRNA homologous to creatine kinase M mRNA have a similar translational regulation during embryogenesis.

Loreni, F., Francesconi, A., Jappelli, R., Amaldi, F. (1992). Analysis of mRNAs under translational control during Xenopus embryogenesis: Isolation of new ribosomal protein clones. NUCLEIC ACIDS RESEARCH, 20(8), 1859-1863 [10.1093/nar/20.8.1859].

Analysis of mRNAs under translational control during Xenopus embryogenesis: Isolation of new ribosomal protein clones

LORENI, FABRIZIO;
1992-01-01

Abstract

We have analyzed several randomly selected mRNAs, of the relatively abundant category, on the basis of maternal or zygotic origin and translational efficiency at different developmental stages. For this purpose, clones from a Xenopus embryo cDNA library were hybridized with cDNA probes prepared with poly(A) + RNA from polysomes and from mRNPs of embryos at different stages. The results obtained indicate that the majority of the relatively abundant MRNAs (38 out of 61) is subject to some kind of translational regulation during embryogenesis. Moreover, 30 clones have been selected as corresponding to mRNAs that behave, from the point of view of transcriptional and translational regulation, similarly to previously studied ribosomal protein (r-protein) mRNAs. Sequence analysis of 20 of these selected cDNAs has shown that half of them are in fact homologous to already sequenced r-protein mRNAs. Unexpectedly we have found that also the mRNA for alpha-cardiac actin and another mRNA homologous to creatine kinase M mRNA have a similar translational regulation during embryogenesis.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/11
English
alpha actin; creatine kinase; messenger rna; ribosome protein; animal cell; article; embryo; embryo development; molecular cloning; nonhuman; nucleotide sequence; polysome; priority journal; rna analysis; translation regulation; xenopus laevis; Actins; Amino Acid Sequence; Animal; Base Sequence; Blotting, Northern; Cloning, Molecular; Creatine Kinase; DNA Probes; Embryo, Nonmammalian; Gene Expression Regulation; Molecular Sequence Data; Ribosomal Proteins; RNA, Messenger; Support, Non-U.S. Gov't; Translation, Genetic; Xenopus laevis
Loreni, F., Francesconi, A., Jappelli, R., Amaldi, F. (1992). Analysis of mRNAs under translational control during Xenopus embryogenesis: Isolation of new ribosomal protein clones. NUCLEIC ACIDS RESEARCH, 20(8), 1859-1863 [10.1093/nar/20.8.1859].
Loreni, F; Francesconi, A; Jappelli, R; Amaldi, F
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/44150
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 12
social impact