The short-term evolution of micronuclei derived from acentric fragments and whole chromosomes was studied in root tips of Vicia faba. Micronuclei were induced by X-rays (30 cGy and 120 cGy) and colchicine (10(-5) M and 3 X 10(-4) M). Frequencies of chromosome breakage or loss of micronuclei in interphase and mitotic cells were studied. The DNA content of micronuclei in interphase cells was also measured. Micronuclei derived from whole chromosome showed a higher probability to survive and to undergo mitotic condensation in synchrony with main nuclei than micronuclei derived from an acentric fragment. PCC (Premature Chromosome Condensation) was not observed for both types of micronuclei in Vicia faba, in contrast to the ones reported in mammalian cells in culture.
Gustavino, B., Vitagliano, E., Sottili, A., Rizzoni, M. (1987). A comparison between short-term evolution of micronuclei induced by X-rays and colchicine in root tips of Vicia faba. 24. Gustavino B, Vitagliano E, Sottili A, Rizzoni M. A comparison between short-term evolution of micronuclei induced by X-rays and colchicine in root tips of Vicia faba. MUTATION RESEARCH, 192(2), 109-119 [doi:10.1016/0165-7992(87)90106-0].
A comparison between short-term evolution of micronuclei induced by X-rays and colchicine in root tips of Vicia faba. 24. Gustavino B, Vitagliano E, Sottili A, Rizzoni M. A comparison between short-term evolution of micronuclei induced by X-rays and colchicine in root tips of Vicia faba.
GUSTAVINO, BIANCA;RIZZONI, MARCO
1987-01-01
Abstract
The short-term evolution of micronuclei derived from acentric fragments and whole chromosomes was studied in root tips of Vicia faba. Micronuclei were induced by X-rays (30 cGy and 120 cGy) and colchicine (10(-5) M and 3 X 10(-4) M). Frequencies of chromosome breakage or loss of micronuclei in interphase and mitotic cells were studied. The DNA content of micronuclei in interphase cells was also measured. Micronuclei derived from whole chromosome showed a higher probability to survive and to undergo mitotic condensation in synchrony with main nuclei than micronuclei derived from an acentric fragment. PCC (Premature Chromosome Condensation) was not observed for both types of micronuclei in Vicia faba, in contrast to the ones reported in mammalian cells in culture.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
