Contryphan-Vn is a D-tryptophan-containing disulfide-constrained nonapeptide isolated from the venom of Conus ventricosus, the single Mediterranean cone snail species. The structure of the synthetic Contryphan-Vn has been determined by NMR spectroscopy. Unique among Contryphans, Contryphan-Vn displays the peculiar presence of a Lys-Trp dyad, reminiscent of that observed in several voltage-gated K+ channel blockers. Electrophysiological experiments carried out on dorsal unpaired median neurons isolated from the cockroach (Periplaneta americana) nerve cord on rat fetal chromaffin cells indicate that Contryphan-Vn affects both voltage-gated and Ca2+-dependent K+ channel activities, with composite and diversified effects in invertebrate and vertebrate systems. Voltage-gated and Ca2+-dependent K+ channels represent the first functional target identified for a conopeptide of the Contryphan family. Furthermore, Contryphan-Vn is the first conopeptide known to modulate the activity of Ca2+-dependent K+ channels. © 2003 Elsevier Science (USA). All rights reserved.

Raybaudi Massilia, G., Eliseo, T., Grolleau, F., Lapied, B., Barbier, J., Bournaud, R., et al. (2003). Contryphan-Vn: A modulator of Ca2+-dependent K+ channels. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 303(1), 238-246 [10.1016/S0006-291X(03)00331-0].

Contryphan-Vn: A modulator of Ca2+-dependent K+ channels

CICERO, DANIEL OSCAR;PACI, MAURIZIO;
2003-01-01

Abstract

Contryphan-Vn is a D-tryptophan-containing disulfide-constrained nonapeptide isolated from the venom of Conus ventricosus, the single Mediterranean cone snail species. The structure of the synthetic Contryphan-Vn has been determined by NMR spectroscopy. Unique among Contryphans, Contryphan-Vn displays the peculiar presence of a Lys-Trp dyad, reminiscent of that observed in several voltage-gated K+ channel blockers. Electrophysiological experiments carried out on dorsal unpaired median neurons isolated from the cockroach (Periplaneta americana) nerve cord on rat fetal chromaffin cells indicate that Contryphan-Vn affects both voltage-gated and Ca2+-dependent K+ channel activities, with composite and diversified effects in invertebrate and vertebrate systems. Voltage-gated and Ca2+-dependent K+ channels represent the first functional target identified for a conopeptide of the Contryphan family. Furthermore, Contryphan-Vn is the first conopeptide known to modulate the activity of Ca2+-dependent K+ channels. © 2003 Elsevier Science (USA). All rights reserved.
2003
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Ca2+ -dependent K+ channels; Cockroach dorsal unpaired median neurons; Contryphan-Vn; Rat fetal chromaffin cells; Solution structure
Raybaudi Massilia, G., Eliseo, T., Grolleau, F., Lapied, B., Barbier, J., Bournaud, R., et al. (2003). Contryphan-Vn: A modulator of Ca2+-dependent K+ channels. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 303(1), 238-246 [10.1016/S0006-291X(03)00331-0].
Raybaudi Massilia, G; Eliseo, T; Grolleau, F; Lapied, B; Barbier, J; Bournaud, R; Molgo, J; Cicero, Do; Paci, M; Schinina, M; Ascenzi, P; Polticelli, ...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/43577
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