Nitric oxide (NO) is a polypotent regulatory molecule involved in a variety of activities, such as the modulation of the catalytic activity of cysteine-containing enzymes. The present study reports the modulation of the HIV-1 reverse transcriptase activity by NO, released by the NO donors 3,3-bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene (NOC-18), (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]5-nitro-3-hexenamide (NOR-3), 3-morpholinosydnonimine (SIN-1), 4-(phenylsulfonyl)-3-((2-(dimethylamino) ethyl)thio)furoxan oxalate (SNO-102), and sodium nitroprusside (SNP). NO inhibits dose-dependently the HIV-1 reverse transcriptase activity, Likely due to oxidation of Cys residue(s). Present results, representing a new insight into the modulation mechanism of the HIV-1 reverse transcriptase activity, may be relevant to develop new strategies for inhibition of HIV-1 replication. (C) 1999 Academic Press.
Persichini, T., Colasanti, M., Fraziano, M., Colizzi, V., Medana, C., Polticelli, F., et al. (1999). Nitric oxide inhibits the HIV-1 reverse transcriptase activity. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 258(3), 624-627.
Tipologia: | Articolo su rivista |
Citazione: | Persichini, T., Colasanti, M., Fraziano, M., Colizzi, V., Medana, C., Polticelli, F., et al. (1999). Nitric oxide inhibits the HIV-1 reverse transcriptase activity. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 258(3), 624-627. |
IF: | Con Impact Factor ISI |
Lingua: | English |
Settore Scientifico Disciplinare: | Settore MED/04 - Patologia Generale Settore BIO/10 |
Revisione (peer review): | Sì, ma tipo non specificato |
Tipo: | Articolo |
Rilevanza: | Rilevanza internazionale |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1006/bbrc.1999.0581 |
Stato di pubblicazione: | Pubblicato |
Data di pubblicazione: | 1999 |
Titolo: | Nitric oxide inhibits the HIV-1 reverse transcriptase activity |
Autori: | |
Autori: | Persichini, T; Colasanti, M; Fraziano, M; Colizzi, V; Medana, C; Polticelli, F; Venturini, G; Ascenzi, P |
Appare nelle tipologie: | 01 - Articolo su rivista |