In this study, a sterile and biocompatible chitosan (CHI) A gel for wound healing applications was formulated. CHI powder was treated in an autoclave (ttCHI) to prepare sterile formulations. The heat treatment modified the CHI molecular weight, as evidenced by GPC analysis, and its physical– chemical features. Differential scanning calorimetry studies indicated that the macromolecules, before and after thermal treatment, differ in the strength of water-polymer interaction leading to different viscoelastic and flowproperties. Thermally treated CHI exhibited the following effects: (i) increased the proliferation and migration of human foreskin foetal fibroblasts at 24 h; (ii) accelerated wound healing (measured as area of lesion) at 3 and 10 days in an in vivo model of pressure ulcers. These effectswere linked to the increase of the hydroxyproline and haemoglobin content as well as Wnt protein expression. Moreover, we found a reduction of myeloperoxidase activity and TNF-α mRNA expression. These observations suggest the potential of this novel CHI gel in wound healing and other therapeutic applications.
Laura, M., Daniela De, S., Eleonora, F., Francesca De, F., Claudia, C., Matricardi, P., et al. (2014). Design and Characterization of a Chitosan Physical Gel Promoting Wound Healing in Mice. JOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE(25), 1483-1493 [10.1007/s10856-014-5175-7].
Design and Characterization of a Chitosan Physical Gel Promoting Wound Healing in Mice
MATRICARDI, PIETRO;
2014-01-01
Abstract
In this study, a sterile and biocompatible chitosan (CHI) A gel for wound healing applications was formulated. CHI powder was treated in an autoclave (ttCHI) to prepare sterile formulations. The heat treatment modified the CHI molecular weight, as evidenced by GPC analysis, and its physical– chemical features. Differential scanning calorimetry studies indicated that the macromolecules, before and after thermal treatment, differ in the strength of water-polymer interaction leading to different viscoelastic and flowproperties. Thermally treated CHI exhibited the following effects: (i) increased the proliferation and migration of human foreskin foetal fibroblasts at 24 h; (ii) accelerated wound healing (measured as area of lesion) at 3 and 10 days in an in vivo model of pressure ulcers. These effectswere linked to the increase of the hydroxyproline and haemoglobin content as well as Wnt protein expression. Moreover, we found a reduction of myeloperoxidase activity and TNF-α mRNA expression. These observations suggest the potential of this novel CHI gel in wound healing and other therapeutic applications.| File | Dimensione | Formato | |
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