The development of convenient, real-time probes for monitoring protein function in biological samples represents an important challenge of the postgenomic era. In response, we introduce here "transcription factor beacons," binding-activated fluorescent DNA probes that signal the presence of specific DNA-binding activities. As a proof of principle, we present beacons for the rapid, sensitive detection of three transcription factors (TATA Binding Protein, Myc-Max, and NF-κB), and measure binding activity directly in crude nuclear extracts.

Vallée_bélisle, A., Bonham, A., Reich, N., Ricci, F., Plaxco, K. (2011). Transcription Factor Beacons for the Quantitative Detection of DNA Binding Activity. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(35), 13836-13839 [10.1021/ja204775k].

Transcription Factor Beacons for the Quantitative Detection of DNA Binding Activity

RICCI, FRANCESCO;
2011-01-01

Abstract

The development of convenient, real-time probes for monitoring protein function in biological samples represents an important challenge of the postgenomic era. In response, we introduce here "transcription factor beacons," binding-activated fluorescent DNA probes that signal the presence of specific DNA-binding activities. As a proof of principle, we present beacons for the rapid, sensitive detection of three transcription factors (TATA Binding Protein, Myc-Max, and NF-κB), and measure binding activity directly in crude nuclear extracts.
2011
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore CHIM/01 - CHIMICA ANALITICA
English
Con Impact Factor ISI
Vallée_bélisle, A., Bonham, A., Reich, N., Ricci, F., Plaxco, K. (2011). Transcription Factor Beacons for the Quantitative Detection of DNA Binding Activity. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(35), 13836-13839 [10.1021/ja204775k].
Vallée_bélisle, A; Bonham, A; Reich, N; Ricci, F; Plaxco, K
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/42274
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