A major problem in assessing the role of calpains in apoptosis induction concerns the fact that calpain inhibitors can also impair the activity of the proteasome, also reported to be involved in apoptosis. Herein we showed that apoptosis induced by calphostin C in U937 human promonocytic leukemia cells was associated, at its onset, with enhanced protein (poly)ubiquitination. This observation prompted us to study whether protein degradation through the ubiquitin/proteasome pathway was involved in apoptosis induction. We found that N-acetyl-Leu-Leu-norleucinal (50 microM), a proteasome as well as a calpain inhibitor, was able to reduce calphostin C-induced apoptosis by approximately 60%, whereas lactacystin (10 microM), a specific proteasome inhibitor, was ineffective. These results suggest that calphostin C-induced apoptosis is partly calpain-mediated, but does not require protein degradation through the ubiquitin/proteasome pathway.
Spinedi, A., Oliverio, S., Di Sano, F., & Piacentini, M. (1998). Calpain involvement in calphostin C-induced apoptosis. BIOCHEMICAL PHARMACOLOGY, 56(11), 1489-1492.
|Tipologia:||Articolo su rivista|
|Citazione:||Spinedi, A., Oliverio, S., Di Sano, F., & Piacentini, M. (1998). Calpain involvement in calphostin C-induced apoptosis. BIOCHEMICAL PHARMACOLOGY, 56(11), 1489-1492.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/06|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/S0006-2952(98)00169-5|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||1-dic-1998|
|Titolo:||Calpain involvement in calphostin C-induced apoptosis|
|Autori:||Spinedi, A; Oliverio, S; Di Sano, F; Piacentini, M|
|Appare nelle tipologie:||01 - Articolo su rivista|