One of the major issues concerning human skeletal muscle progenitor cells is represented by the efficient isolation and in vitro expansion of cells retaining the ability to proliferate, migrate and differentiate once transplanted. Here we describe a method (1) effective in obtaining human muscle precursor cells both from fresh and frozen biopsies coming from different muscles, (2) selective to yield cells uniformly positive for CD56 and negative for CD34 without FACS sorting, (3) reliable in maintaining proliferative and in vitro differentiative capacity up to passage 10.
Franzin, C., Piccoli, M., Urbani, L., Biz, C., Gamba, P., DE COPPI, P., et al. (2016). Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies. In Stem Cell Heterogeneity. Methods and Protocols. METHODS IN MOLECULAR BIOLOGY [10.1007/7651_2016_321].
Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies
DE COPPI, PAOLO;
2016-01-01
Abstract
One of the major issues concerning human skeletal muscle progenitor cells is represented by the efficient isolation and in vitro expansion of cells retaining the ability to proliferate, migrate and differentiate once transplanted. Here we describe a method (1) effective in obtaining human muscle precursor cells both from fresh and frozen biopsies coming from different muscles, (2) selective to yield cells uniformly positive for CD56 and negative for CD34 without FACS sorting, (3) reliable in maintaining proliferative and in vitro differentiative capacity up to passage 10.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
