Amniotic fluid-derived stem (AFS) cells have been described as an appealing source of stem cells because of their (1) fetal, non-embryonic origin, (2) easy access during pregnancy overcoming the ethical issues related both to the use of human embryonic cells and to the postnatal tissue biopsy with donor site mor- bidity, and (3) their undemanding ability to be expanded. We and others have demonstrated the broad differentiation potential and here we describe the established protocol we developed to obtain c-Kit+ human AFS cells, starting from second trimester amniocentesis samples.
Pozzobon, M., Piccoli, M., Schiavo, A.a., Atala, A., DE COPPI, P. (2013). Isolation of c-Kit+ human amniotic fluid stem cells from second trimester. In Methods in Molecular Biology (pp. 191-198). Humana Press Inc. [10.1007/978-1-62703-508-8_16].
Isolation of c-Kit+ human amniotic fluid stem cells from second trimester
DE COPPI, PAOLO
2013-01-01
Abstract
Amniotic fluid-derived stem (AFS) cells have been described as an appealing source of stem cells because of their (1) fetal, non-embryonic origin, (2) easy access during pregnancy overcoming the ethical issues related both to the use of human embryonic cells and to the postnatal tissue biopsy with donor site mor- bidity, and (3) their undemanding ability to be expanded. We and others have demonstrated the broad differentiation potential and here we describe the established protocol we developed to obtain c-Kit+ human AFS cells, starting from second trimester amniocentesis samples.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
