Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Here, we produced iPS cells from mouse amniotic fluid cells, using a non-viral-based transposon system. All obtained iPS cell lines exhibited characteristics of pluripotent cells, including the ability to differentiate toward derivatives of all three germ layers in vitro and in vivo. This strategy opens up the possibility of using cells from diseased fetuses to develop new therapies for birth defects.

Bertin, E., Piccoli, M., Franzin, C., Nagy, A., Mileikovsky, M., De Coppi, P., et al. (2017). The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System. JOURNAL OF VISUALIZED EXPERIMENTS(120) [10.3791/54598].

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

De Coppi, Paolo;
2017-01-01

Abstract

Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Here, we produced iPS cells from mouse amniotic fluid cells, using a non-viral-based transposon system. All obtained iPS cell lines exhibited characteristics of pluripotent cells, including the ability to differentiate toward derivatives of all three germ layers in vitro and in vivo. This strategy opens up the possibility of using cells from diseased fetuses to develop new therapies for birth defects.
2017
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIOS-04/A - Anatomia, biologia cellulare e biologia dello sviluppo comparate
English
Amniotic Fluid
Animals
Cell Differentiation
Cell Line
Cellular Reprogramming
Female
Induced Pluripotent Stem Cells
Mice
Pregnancy
Pregnancy
Animal
Bertin, E., Piccoli, M., Franzin, C., Nagy, A., Mileikovsky, M., De Coppi, P., et al. (2017). The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System. JOURNAL OF VISUALIZED EXPERIMENTS(120) [10.3791/54598].
Bertin, E; Piccoli, M; Franzin, C; Nagy, A; Mileikovsky, M; De Coppi, P; Pozzobon, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/417806
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