Biofilm is common bacterial life-style and it plays a crucial role in human health as biofilm21 mediated infections. Recently to counteract biofilm development new nano-structured biomaterials 22 have been proposed. The susceptibility of nano-structured materials to microbial colonization and 23 biofilm formation has not been thoroughly considered. Here, the ability of Streptococcus mutans 24 and Pseudomonas aeruginosa to colonize surfaces coated with single wall carbon nano-tubes 25 (SWCNTs) was analysed by BioTimer Assay (BTA). 26 Results showed that BTA was reliable to evaluate the sterility of glass beads coated with SWCNTs 27 (SWCNTs-GBs) as well as to quantify the bacterial adhesion and biofilm development showing that 28 SWCNTs-GBs were colonized at the same extent of uncoated GBs both by S. mutans and P. 29 aeruginosa. Atomic Force Microscopy was used to visualize adherent bacteria and biofilm on 30 SWCNTs-GBs. 31 In conclusion, our results demonstrate that single wall SWCNTs-coated surfaces are not suitable to 32 counteract bacterial adhesion and biofilm development.
Pantanella, F., Berlutti, F., Passer, D., Sordi, D., Frioni, A., TERRANOVA PERSICHELLI, M.l., et al. (2011). Quantitative evaluation of bacteria adherent and in biofilm on single wall carbon nanotube coated surface. INTERDISCIPLINARY PERSPECTIVES ON INFECTIOUS DISEASES, 2011, 291513-291519 [10.1155/2011/291513].
Quantitative evaluation of bacteria adherent and in biofilm on single wall carbon nanotube coated surface
TERRANOVA PERSICHELLI, MARIA LETIZIA;
2011-01-01
Abstract
Biofilm is common bacterial life-style and it plays a crucial role in human health as biofilm21 mediated infections. Recently to counteract biofilm development new nano-structured biomaterials 22 have been proposed. The susceptibility of nano-structured materials to microbial colonization and 23 biofilm formation has not been thoroughly considered. Here, the ability of Streptococcus mutans 24 and Pseudomonas aeruginosa to colonize surfaces coated with single wall carbon nano-tubes 25 (SWCNTs) was analysed by BioTimer Assay (BTA). 26 Results showed that BTA was reliable to evaluate the sterility of glass beads coated with SWCNTs 27 (SWCNTs-GBs) as well as to quantify the bacterial adhesion and biofilm development showing that 28 SWCNTs-GBs were colonized at the same extent of uncoated GBs both by S. mutans and P. 29 aeruginosa. Atomic Force Microscopy was used to visualize adherent bacteria and biofilm on 30 SWCNTs-GBs. 31 In conclusion, our results demonstrate that single wall SWCNTs-coated surfaces are not suitable to 32 counteract bacterial adhesion and biofilm development.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
