Many studies suggest that endoplasmic reticulum (ER) Ca2+ pool rather than cytosolic Ca2+ may play a crucial role in triggering apoptosis. In this study, we performed an image analysis of cells loaded with the fluorescent dye chlortetracycline (CTC) to in situ analyze Ca2+ changes within the ER in apoptosing promonocytic U937 cells. The results, validated through the use of thapsigargin (THG) as ER Ca2+ depletor, confirm the findings that apoptotic cells have a Ca2+-depleted ER, in contrast with treated but still viable cells.
Cerella, C., Mearelli, C., De Nicola, M., D'Alessio, M., Magrini, A., Bergamaschi, A., et al. (2007). Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1099, 490-493 [10.1196/annals.1387.021].
Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline
MAGRINI, ANDREA;GHIBELLI, LINA
2007-03-01
Abstract
Many studies suggest that endoplasmic reticulum (ER) Ca2+ pool rather than cytosolic Ca2+ may play a crucial role in triggering apoptosis. In this study, we performed an image analysis of cells loaded with the fluorescent dye chlortetracycline (CTC) to in situ analyze Ca2+ changes within the ER in apoptosing promonocytic U937 cells. The results, validated through the use of thapsigargin (THG) as ER Ca2+ depletor, confirm the findings that apoptotic cells have a Ca2+-depleted ER, in contrast with treated but still viable cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
