Epstein-Barr Virus (EBV) is involved in the progression of lymphomas through still unknown mechanism involving increased resistance to induced apoptosis. We show here that in a set of apoptosis-resistant EBV-converted Burkitt's lymphoma clones, 5- and 12-lipoxygenases (LOXs) are over-expressed. Further investigations on 5-LOX showed that resistance to apoptosis increases parallely with the expression of 5-lipoxygenase (5-LOX). Inhibitors of 5-LOX: (a) decrease peroxides level, indicating that this enzyme promotes the generation of oxidative stress in EBV+ cells, and (b) potently induce apoptosis in the EBV resistant cell line E2R. 5- and 15-HETE, the products of the 5 and 15-LOXs, respectively, counteract 5-LOX inhibitor induced apoptosis, indicating that products of arachidonate metabolism, rather than peroxides, trigger a signal transduction that is required for survival of the EBV-converted cells. These findings suggest that 5- and, to a lesser extent, other LOXs, that are involved in tumor progression of several cell types, may also participate in lymphomagenesis, especially that EBV-mediated.

Belfiore, M., Natoni, A., Barzellotti, R., Merendino, N., Pessina, G., Ghibelli, L., et al. (2007). Involvement of 5-lipoxygenase in survival of Epstein-Barr virus (EBV)-converted B lymphoma cells. CANCER LETTERS, 254(2), 236-243 [10.1016/j.canlet.2007.03.010].

Involvement of 5-lipoxygenase in survival of Epstein-Barr virus (EBV)-converted B lymphoma cells

GHIBELLI, LINA;
2007-09-08

Abstract

Epstein-Barr Virus (EBV) is involved in the progression of lymphomas through still unknown mechanism involving increased resistance to induced apoptosis. We show here that in a set of apoptosis-resistant EBV-converted Burkitt's lymphoma clones, 5- and 12-lipoxygenases (LOXs) are over-expressed. Further investigations on 5-LOX showed that resistance to apoptosis increases parallely with the expression of 5-lipoxygenase (5-LOX). Inhibitors of 5-LOX: (a) decrease peroxides level, indicating that this enzyme promotes the generation of oxidative stress in EBV+ cells, and (b) potently induce apoptosis in the EBV resistant cell line E2R. 5- and 15-HETE, the products of the 5 and 15-LOXs, respectively, counteract 5-LOX inhibitor induced apoptosis, indicating that products of arachidonate metabolism, rather than peroxides, trigger a signal transduction that is required for survival of the EBV-converted cells. These findings suggest that 5- and, to a lesser extent, other LOXs, that are involved in tumor progression of several cell types, may also participate in lymphomagenesis, especially that EBV-mediated.
8-set-2007
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/13 - BIOLOGIA APPLICATA
English
Con Impact Factor ISI
Reactive Oxygen Species; Burkitt Lymphoma; Lipid Peroxidation; Arachidonate 12-Lipoxygenase; RNA, Neoplasm; Humans; Cell Line, Tumor; Cell Survival; Apoptosis; Disease Progression; Herpesvirus 4, Human; Arachidonate 5-Lipoxygenase; Reverse Transcriptase Polymerase Chain Reaction
Belfiore, M., Natoni, A., Barzellotti, R., Merendino, N., Pessina, G., Ghibelli, L., et al. (2007). Involvement of 5-lipoxygenase in survival of Epstein-Barr virus (EBV)-converted B lymphoma cells. CANCER LETTERS, 254(2), 236-243 [10.1016/j.canlet.2007.03.010].
Belfiore, M; Natoni, A; Barzellotti, R; Merendino, N; Pessina, G; Ghibelli, L; Gualandi, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/41539
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