A protein kinase activity associated with maize root plasma membranes was partially purified and characterized. Biochemical properties, such as calcium dependence, inhibition by calmodulin antagonists, and absence of calmodulin stimulation; indicated that the enzyme belongs to the calcium-dependent protein kinase (CDPK) family. By means of an in-gel phosphorylation assay the molecular mass of active polypeptides was determined: two bands of 55 and 51 kDa became labelled. The same proteins were also immunodecorated by monoclonal antibodies against soybean CDPK. Results from in vitro assays demonstrated that maize H+-ATPase was a suitable substrate for this protein kinase and that the phosphorylation site was located at the C-terminal domain of the enzyme. This result was confirmed by using as substrate in phosphorylation assays the isolated C-terminal domain of the H+- ATPase expressed in Escherichia coli as a glutathione-transferase fusion protein.
Camoni, L., Fullone, M.R., Marra, M., & Aducci, P. (1998). The plasma membrane H+-ATPase from maize roots is phosphorylated in the C-terminal domain by a calcium-dependent protein kinase. PHYSIOLOGIA PLANTARUM, 104(4), 549-555.
Tipologia: | Articolo su rivista | |
Citazione: | Camoni, L., Fullone, M.R., Marra, M., & Aducci, P. (1998). The plasma membrane H+-ATPase from maize roots is phosphorylated in the C-terminal domain by a calcium-dependent protein kinase. PHYSIOLOGIA PLANTARUM, 104(4), 549-555. | |
Altre informazioni significative: | 7 | |
IF: | Con Impact Factor ISI | |
Lingua: | English | |
Settore Scientifico Disciplinare: | Settore BIO/04 | |
Revisione (peer review): | Sì, ma tipo non specificato | |
Tipo: | Articolo | |
Rilevanza: | Rilevanza internazionale | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1034/j.1399-3054.1998.1040405.x | |
Stato di pubblicazione: | Pubblicato | |
Data di pubblicazione: | 1998 | |
Titolo: | The plasma membrane H+-ATPase from maize roots is phosphorylated in the C-terminal domain by a calcium-dependent protein kinase | |
Autori: | ||
Autori: | Camoni, L; Fullone, MR; Marra, M; Aducci, P | |
Appare nelle tipologie: | 01 - Articolo su rivista |