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EnglishA protein kinase activity associated with maize root plasma membranes was partially purified and characterized. Biochemical properties, such as calcium dependence, inhibition by calmodulin antagonists, and absence of calmodulin stimulation; indicated that the enzyme belongs to the calcium-dependent protein kinase (CDPK) family. By means of an in-gel phosphorylation assay the molecular mass of active polypeptides was determined: two bands of 55 and 51 kDa became labelled. The same proteins were also immunodecorated by monoclonal antibodies against soybean CDPK. Results from in vitro assays demonstrated that maize H+-ATPase was a suitable substrate for this protein kinase and that the phosphorylation site was located at the C-terminal domain of the enzyme. This result was confirmed by using as substrate in phosphorylation assays the isolated C-terminal domain of the H+- ATPase expressed in Escherichia coli as a glutathione-transferase fusion protein.
| Tipologia: | Articolo su rivista |
| Citazione: | Camoni, L., Fullone, M.R., Marra, M., & Aducci, P. (1998). The plasma membrane H+-ATPase from maize roots is phosphorylated in the C-terminal domain by a calcium-dependent protein kinase. PHYSIOLOGIA PLANTARUM, 104(4), 549-555. |
| Altre informazioni significative: | 7 |
| IF: | Con Impact Factor ISI |
| Lingua: | English |
| Settore Scientifico Disciplinare: | Settore BIO/04 |
| Revisione (peer review): | Sì, ma tipo non specificato |
| Tipo: | Articolo |
| Rilevanza: | Rilevanza internazionale |
| Digital Object Identifier (DOI): | 10.1034/j.1399-3054.1998.1040405.x |
| Stato di pubblicazione: | Pubblicato |
| Data di pubblicazione: | 1998 |
| Titolo: | The plasma membrane H+-ATPase from maize roots is phosphorylated in the C-terminal domain by a calcium-dependent protein kinase |
| Autori: | |
| Autori: | Camoni, L; Fullone, MR; Marra, M; Aducci, P |
| Appare nelle tipologie: | 01 - Articolo su rivista |
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