We have previously identified 12 surface antigens whose differential expression represented the signature of B-cell chronic lymphocytic leukemia (B-CLL) subsets with different prognosis. In the present study, expression data for these antigens, as determined in 137 B-CLL cases, all with survivals, were utilized to devise a comprehensive immunophenotypic scoring system of prognostic relevance for B-CLL patients. In particular, univariate z score was employed to identify the markers with greater prognostic impact, while maximally selected log-rank statistics were chosen to define the optimal cut-off points capable to split patients into two groups with different survivals. A weighted immunophenotypic scoring system was developed by integrating results from these analyses. Six antigens were selected: three positive prognosticators (CD62L, CD54, CD49c) and three negative prognosticators (CD49d, CD38, CD79b), with cut-off values ranging from 30% to 50% of positive cells. By weighing the expression of each marker according to its statistical power, a complete scoring system, with point values comprised between 0 (complete absence of phenotypic conditions associated with good prognosis) and 9 (all the phenotypic conditions associated with good prognosis fulfilled), allowed to split the whole set of B-CLL patients, into three distinctive prognostic groups (P = 4.78 x 10(-11)) with high- (score 0-3), intermediate- (score 4-6), and low- (score 7-9) risk of death. The three risk groups showed different distribution of cases as for Rai's stages, IgVH mutations, and ZAP-70 expression. The proposed immunophenotypic scoring system may be an additional useful tool in routine diagnostic/prognostic procedures for B-CLL.

Zucchetto, A., Bomben, R., Dal Bo, M., Sonego, P., Nanni, P., Rupolo, M., et al. (2006). A scoring system based on the expression of six surface molecules allows the identification of three prognostic risk groups in B-cell chronic lymphocytic leukemia. JOURNAL OF CELLULAR PHYSIOLOGY, 207(2), 354-363 [10.1002/jcp.20570].

A scoring system based on the expression of six surface molecules allows the identification of three prognostic risk groups in B-cell chronic lymphocytic leukemia

DEL POETA, GIOVANNI;DEL PRINCIPE, MARIA ILARIA;
2006-05-01

Abstract

We have previously identified 12 surface antigens whose differential expression represented the signature of B-cell chronic lymphocytic leukemia (B-CLL) subsets with different prognosis. In the present study, expression data for these antigens, as determined in 137 B-CLL cases, all with survivals, were utilized to devise a comprehensive immunophenotypic scoring system of prognostic relevance for B-CLL patients. In particular, univariate z score was employed to identify the markers with greater prognostic impact, while maximally selected log-rank statistics were chosen to define the optimal cut-off points capable to split patients into two groups with different survivals. A weighted immunophenotypic scoring system was developed by integrating results from these analyses. Six antigens were selected: three positive prognosticators (CD62L, CD54, CD49c) and three negative prognosticators (CD49d, CD38, CD79b), with cut-off values ranging from 30% to 50% of positive cells. By weighing the expression of each marker according to its statistical power, a complete scoring system, with point values comprised between 0 (complete absence of phenotypic conditions associated with good prognosis) and 9 (all the phenotypic conditions associated with good prognosis fulfilled), allowed to split the whole set of B-CLL patients, into three distinctive prognostic groups (P = 4.78 x 10(-11)) with high- (score 0-3), intermediate- (score 4-6), and low- (score 7-9) risk of death. The three risk groups showed different distribution of cases as for Rai's stages, IgVH mutations, and ZAP-70 expression. The proposed immunophenotypic scoring system may be an additional useful tool in routine diagnostic/prognostic procedures for B-CLL.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/15 - Malattie del Sangue
English
Con Impact Factor ISI
Intercellular Adhesion Molecule-1; L-Selectin; Humans; Integrin alpha4; Prognosis; Aged; Antigens, CD38; Antigens, CD79; Multivariate Analysis; Antigens, CD19; Leukemia, Lymphocytic, Chronic, B-Cell; Aged, 80 and over; Risk Factors; Antigens, CD; Adult; Integrin alpha3; Middle Aged; Flow Cytometry; Immunophenotyping; Male; Female; Survival Analysis; Proportional Hazards Models
Zucchetto, A., Bomben, R., Dal Bo, M., Sonego, P., Nanni, P., Rupolo, M., et al. (2006). A scoring system based on the expression of six surface molecules allows the identification of three prognostic risk groups in B-cell chronic lymphocytic leukemia. JOURNAL OF CELLULAR PHYSIOLOGY, 207(2), 354-363 [10.1002/jcp.20570].
Zucchetto, A; Bomben, R; Dal Bo, M; Sonego, P; Nanni, P; Rupolo, M; Bulian, P; Dal Maso, L; DEL POETA, G; DEL PRINCIPE, Mi; Degan, M; Gattei, V
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/40669
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