The plant lectins derived from Galanthus nivalis (Snowdrop) (GNA) and Hippeastrum hybrid (Amaryllis) (HHA) selectively inhibited a wide variety of human immunodeficiency virus type 1 (HIV-1) and HIV-2 strains and clinical (CXCR4- and CCR5-using) isolates in different cell types. They also efficiently inhibited infection of T lymphocytes by a variety of mutant virus strains. GNA and HHA markedly prevented syncytium formation between persistently infected HUT-78/HIV cells and uninfected T lymphocytes. The plant lectins did not measurably affect the antiviral activity of other clinically approved anti-HIV drugs used in the clinic when combined with these drugs. Short exposure of the lectins to cell-free virus particles or persistently HIV-infected HUT-78 cells markedly decreased HIV infectivity and increased the protective (microbicidal) activity of the plant lectins. Flow cytometric analysis and monoclonal antibody binding studies and a PCR-based assay revealed that GNA and HHA do not interfere with CD4, CXCR4, CCR5, and DC-SIGN and do not specifically bind with the membrane of uninfected cells. Instead, GNA and HHA likely interrupt the virus entry process by interfering with the virus envelope glycoprotein. HHA and GNA are odorless, colorless, and tasteless, and they are not cytotoxic, antimetabolically active, or mitogenic to human primary T lymphocytes at concentrations that exceed their antivirally active concentrations by 2 to 3 orders of magnitude. GNA and HHA proved stable at high temperature (50°C) and low pH (5.0) for prolonged time periods and can be easily formulated in gel preparations for microbicidal use; they did not agglutinate human erythrocytes and were not toxic to mice when administered intravenously.

Balzarini, J., Hatse, S., Vermeire, K., Princen, K., Aquaro, S., Perno, C.f., et al. (2004). Mannose-specific plant lectins from the Amaryllidaceae family qualify as efficient microbicides for prevention of human immunodeficiency virus infection, 48(10), 3858-3870 [10.1128/AAC.48.10.3858-3870.2004].

Mannose-specific plant lectins from the Amaryllidaceae family qualify as efficient microbicides for prevention of human immunodeficiency virus infection

AQUARO, STEFANO;PERNO, CARLO FEDERICO;
2004-01-01

Abstract

The plant lectins derived from Galanthus nivalis (Snowdrop) (GNA) and Hippeastrum hybrid (Amaryllis) (HHA) selectively inhibited a wide variety of human immunodeficiency virus type 1 (HIV-1) and HIV-2 strains and clinical (CXCR4- and CCR5-using) isolates in different cell types. They also efficiently inhibited infection of T lymphocytes by a variety of mutant virus strains. GNA and HHA markedly prevented syncytium formation between persistently infected HUT-78/HIV cells and uninfected T lymphocytes. The plant lectins did not measurably affect the antiviral activity of other clinically approved anti-HIV drugs used in the clinic when combined with these drugs. Short exposure of the lectins to cell-free virus particles or persistently HIV-infected HUT-78 cells markedly decreased HIV infectivity and increased the protective (microbicidal) activity of the plant lectins. Flow cytometric analysis and monoclonal antibody binding studies and a PCR-based assay revealed that GNA and HHA do not interfere with CD4, CXCR4, CCR5, and DC-SIGN and do not specifically bind with the membrane of uninfected cells. Instead, GNA and HHA likely interrupt the virus entry process by interfering with the virus envelope glycoprotein. HHA and GNA are odorless, colorless, and tasteless, and they are not cytotoxic, antimetabolically active, or mitogenic to human primary T lymphocytes at concentrations that exceed their antivirally active concentrations by 2 to 3 orders of magnitude. GNA and HHA proved stable at high temperature (50°C) and low pH (5.0) for prolonged time periods and can be easily formulated in gel preparations for microbicidal use; they did not agglutinate human erythrocytes and were not toxic to mice when administered intravenously.
2004
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
English
Con Impact Factor ISI
anti human immunodeficiency virus agent; CD209 antigen; CD4 antigen; chemokine receptor CCR5; chemokine receptor CXCR5; mannose; monoclonal antibody; plant extract; plant lectin; virus envelope protein; animal cell; antibody combining site; antimicrobial activity; antiviral activity; article; blood clotting; cell free system; clinical trial; drug selectivity; erythrocyte; flow cytometry; Galanthus; human; human cell; Human immunodeficiency virus 1; Human immunodeficiency virus 2; Human immunodeficiency virus infection; Hut 78; lymphoid cell line; mouse; nonhuman; pH; priority journal; real time polymerase chain reaction; T lymphocyte; temperature acclimatization; virus infectivity; virus mutant; Agglutinins; Animals; Anti-HIV Agents; Antibodies, Monoclonal; Calcium Signaling; Cell Division; Cell-Free System; Cells, Cultured; Coculture Techniques; Erythrocytes; Flow Cytometry; Galanthus; Gels; Giant Cells; HIV-1; Humans; Lectins; Liliaceae; Mannose; Mice; Mitogens; Rabbits; Reverse Transcriptase Polymerase Chain Reaction; Temperature
Balzarini, J., Hatse, S., Vermeire, K., Princen, K., Aquaro, S., Perno, C.f., et al. (2004). Mannose-specific plant lectins from the Amaryllidaceae family qualify as efficient microbicides for prevention of human immunodeficiency virus infection, 48(10), 3858-3870 [10.1128/AAC.48.10.3858-3870.2004].
Balzarini, J; Hatse, S; Vermeire, K; Princen, K; Aquaro, S; Perno, Cf; De Clercq, E; Egberink, H; Vanden Mooter, G; Peumans, W; Van Damme, E; Schols, D
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

Questo articolo è pubblicato sotto una Licenza Licenza Creative Commons Creative Commons

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/40646
Citazioni
  • ???jsp.display-item.citation.pmc??? 60
  • Scopus ND
  • ???jsp.display-item.citation.isi??? 129
social impact