In this work we present and analyse XAS measurements carried out on various portions of Prion-protein tetra-octa-repeat peptides in complexes with Cu(II) ions, both in the presence and in the absence of Zn(II). Because of the ability of the XAS technique to provide detailed local structural information, we are able to demonstrate that Zn acts by directly interacting with the peptide, in this way competing with Cu for binding with histidine. This finding suggests that metal binding competition can be important in the more general context of metal homeostasis.
Stellato, F., Spevacek, A., Proux, O., Minicozzi, V., Millhauser, G., Morante, S. (2011). Zinc modulates copper coordination mode in prion protein octa-repeat subdomains. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 40(11), 1259-1270 [10.1007/s00249-011-0713-4].
Zinc modulates copper coordination mode in prion protein octa-repeat subdomains
Stellato, F;MINICOZZI, VELIA;MORANTE, SILVIA
2011-01-01
Abstract
In this work we present and analyse XAS measurements carried out on various portions of Prion-protein tetra-octa-repeat peptides in complexes with Cu(II) ions, both in the presence and in the absence of Zn(II). Because of the ability of the XAS technique to provide detailed local structural information, we are able to demonstrate that Zn acts by directly interacting with the peptide, in this way competing with Cu for binding with histidine. This finding suggests that metal binding competition can be important in the more general context of metal homeostasis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
