A negative association between polymorphism Leu-214 and type-1 thymidine analogue mutations (TAM1) and a positive association with a clinically favorable virological response to thymidine analogue-based combination antiretroviral therapy have been described. In this study, the impact of Leu-214 on replication capacity and resistance to zidovudine (ZDV) of viruses containing TAM1 or TAM2 was determined. Leu-214 decreased the growth rate of viruses bearing Tyr-215, as well as their resistance to ZDV. This observation was confirmed by structural and molecular modeling data, suggesting a regulatory role for Leu-214 in the emergence and phenotypic resistance of TAM1.

Puertas, M., Buzon, M., Artese, A., Alcaro, S., Menendez Arias, L., Perno, C.f., et al. (2009). Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility. JOURNAL OF VIROLOGY, 83(15), 7434-7439 [10.1128/JVI.00487-09].

Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility

PERNO, CARLO FEDERICO;CECCHERINI SILBERSTEIN, FRANCESCA;
2009-08-01

Abstract

A negative association between polymorphism Leu-214 and type-1 thymidine analogue mutations (TAM1) and a positive association with a clinically favorable virological response to thymidine analogue-based combination antiretroviral therapy have been described. In this study, the impact of Leu-214 on replication capacity and resistance to zidovudine (ZDV) of viruses containing TAM1 or TAM2 was determined. Leu-214 decreased the growth rate of viruses bearing Tyr-215, as well as their resistance to ZDV. This observation was confirmed by structural and molecular modeling data, suggesting a regulatory role for Leu-214 in the emergence and phenotypic resistance of TAM1.
ago-2009
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
English
Con Impact Factor ISI
leucine; RNA directed DNA polymerase; zidovudine; antiviral susceptibility; article; controlled study; genetic polymorphism; Human immunodeficiency virus 1; mutational analysis; nonhuman; priority journal; protein structure; site directed mutagenesis; virogenesis; virus replication; Anti-HIV Agents; Cell Line; Drug Resistance, Viral; HIV Infections; HIV Reverse Transcriptase; HIV-1; Humans; Leucine; Mutation; Polymorphism, Genetic; Virus Replication; Zidovudine; Human immunodeficiency virus 1
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2708641/pdf/0487-09.pdf
Puertas, M., Buzon, M., Artese, A., Alcaro, S., Menendez Arias, L., Perno, C.f., et al. (2009). Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility. JOURNAL OF VIROLOGY, 83(15), 7434-7439 [10.1128/JVI.00487-09].
Puertas, M; Buzon, M; Artese, A; Alcaro, S; Menendez Arias, L; Perno, Cf; Clotet, B; CECCHERINI SILBERSTEIN, F; Martinez Picado, J
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/39182
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