Anandamide (AEA) is the most studied member of a new class of lipid mediators, collectively called endocannabinoids. The biological activity of AEA at cannabinoid and noncannabinoid receptors depends on its life span in the extracellular space, which is regulated by a rapid cellular uptake, followed by intracellular degradation by the enzyme AEA hydrolase (fatty acid amide hydrolase). Here, we present the methodological details of the procedures that we have developed to assay fatty acid amide hydrolase activity and to characterize AEA transport through cell membranes in a new ideal ex vivo system like brain synaptosomes.

Fezza, F., Battista, N., Bari, M., Maccarrone, M. (2006). Methods to assay anandamide hydrolysis and transport in synaptosomes, 123, 163-168.

Methods to assay anandamide hydrolysis and transport in synaptosomes.

FEZZA, FILOMENA;
2006-01-01

Abstract

Anandamide (AEA) is the most studied member of a new class of lipid mediators, collectively called endocannabinoids. The biological activity of AEA at cannabinoid and noncannabinoid receptors depends on its life span in the extracellular space, which is regulated by a rapid cellular uptake, followed by intracellular degradation by the enzyme AEA hydrolase (fatty acid amide hydrolase). Here, we present the methodological details of the procedures that we have developed to assay fatty acid amide hydrolase activity and to characterize AEA transport through cell membranes in a new ideal ex vivo system like brain synaptosomes.
2006
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
amide; anandamide; arachidonic acid derivative; animal; article; high performance liquid chromatography; hydrolysis; metabolism; methodology; rat; scintillation counting; synaptosome; transport at the cellular level; Animals; Arachidonic Acids; Biological Transport; Chromatography, High Pressure Liquid; Hydrolysis; Polyunsaturated Alkamides; Rats; Scintillation Counting; Synaptosomes
PMID: 16506407
Fezza, F., Battista, N., Bari, M., Maccarrone, M. (2006). Methods to assay anandamide hydrolysis and transport in synaptosomes, 123, 163-168.
Fezza, F; Battista, N; Bari, M; Maccarrone, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/39144
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