Brown adipose tissue (BAT) is mitochondria rich, enabling high oxidative metabolism for non -shivering thermogenesis. The release of large/small extracellular vesicles (EVs) containing mitochondria or mitochondrial fragments, termed mitoEVs, may support mitochondrial quality control or intercellular communication. We present a protocol to isolate and characterize mito-EVs. We detail steps for BAT processing, cell debris removal, differential centrifugation (dC), and mitoEV analysis by flow cytometry and immunoblotting assays. For complete details on the use and execution of this protocol, please refer to Rosina et al. 1
Tortolici, F., DI BIAGIO, C., Lettieri-Barbato, D., Aquilano, K. (2024). Protocol for detecting mitochondria extracellular vesicles of brown adipose tissue in mice. STAR PROTOCOLS, 5(3) [10.1016/j.xpro.2024.103161].
Protocol for detecting mitochondria extracellular vesicles of brown adipose tissue in mice
Flavia Tortolici;Claudia Di Biagio;Daniele Lettieri-Barbato
Funding Acquisition
;Katia Aquilano
2024-01-01
Abstract
Brown adipose tissue (BAT) is mitochondria rich, enabling high oxidative metabolism for non -shivering thermogenesis. The release of large/small extracellular vesicles (EVs) containing mitochondria or mitochondrial fragments, termed mitoEVs, may support mitochondrial quality control or intercellular communication. We present a protocol to isolate and characterize mito-EVs. We detail steps for BAT processing, cell debris removal, differential centrifugation (dC), and mitoEV analysis by flow cytometry and immunoblotting assays. For complete details on the use and execution of this protocol, please refer to Rosina et al. 1| File | Dimensione | Formato | |
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97. (2024) Tortolici et al_Star Protocol.pdf
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