Copper-zinc superoxide dismutase (SOD1) plays a protective role against the toxicity of superoxide, and studies in Saccharomyces cerevisiae and in Drosophila have suggested an additional role for SOD1 in iron metabolism. We have studied the effect of the modulation of SOD1 levels on iron metabolism in a cultured human glial cell line and in a mouse motoneuronal cell line. We observed that levels of the transferrin receptor and the iron regulatory protein 1 were modulated in response to altered intracellular levels of superoxide dismutase activity, carried either by wild-type SOD1 or by an SOD-active amyotrophic lateral sclerosis (ALS) mutant enzyme, G93A-SOD1, but not by a superoxide dismutase inactive ALS mutant, H46R-SOD1. Ferritin expression was also increased by wild-type SOD1 overexpression, but not by mutant SOD1s. We propose that changes in superoxide levels due to alteration of SOD1 activity affect iron metabolism in glial and neuronal cells from higher eukaryotes and that this may be relevant to diseases of the nervous system.

Danzeisen, R., Achsel, T., Bederke, U., Cozzolino, M., Crosio, C., Ferri, A., et al. (2006). Superoxide dismutase 1 modulates expression of transferrin receptor. JBIC, 11(4), 489-498 [10.1007/s00775-006-0099-4].

Superoxide dismutase 1 modulates expression of transferrin receptor

ROTILIO, GIUSEPPE;CARRI', MARIA TERESA
2006-06-01

Abstract

Copper-zinc superoxide dismutase (SOD1) plays a protective role against the toxicity of superoxide, and studies in Saccharomyces cerevisiae and in Drosophila have suggested an additional role for SOD1 in iron metabolism. We have studied the effect of the modulation of SOD1 levels on iron metabolism in a cultured human glial cell line and in a mouse motoneuronal cell line. We observed that levels of the transferrin receptor and the iron regulatory protein 1 were modulated in response to altered intracellular levels of superoxide dismutase activity, carried either by wild-type SOD1 or by an SOD-active amyotrophic lateral sclerosis (ALS) mutant enzyme, G93A-SOD1, but not by a superoxide dismutase inactive ALS mutant, H46R-SOD1. Ferritin expression was also increased by wild-type SOD1 overexpression, but not by mutant SOD1s. We propose that changes in superoxide levels due to alteration of SOD1 activity affect iron metabolism in glial and neuronal cells from higher eukaryotes and that this may be relevant to diseases of the nervous system.
giu-2006
Pubblicato
Rilevanza internazionale
Abstract
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Animals; Protein Biosynthesis; Superoxide Dismutase; Humans; Receptors, Transferrin; Cell Line, Tumor; Mice; Blotting, Western; Transfection; Actins; RNA Interference; Mutation; Cell Line; Ferritins
Danzeisen, R., Achsel, T., Bederke, U., Cozzolino, M., Crosio, C., Ferri, A., et al. (2006). Superoxide dismutase 1 modulates expression of transferrin receptor. JBIC, 11(4), 489-498 [10.1007/s00775-006-0099-4].
Danzeisen, R; Achsel, T; Bederke, U; Cozzolino, M; Crosio, C; Ferri, A; Frenzel, M; Gralla, E; Huber, L; Ludolph, A; Nencini, M; Rotilio, G; Valentine, J; Carri', Mt
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/38865
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