Herein, we developed a new waste solution-free paper-based electrochemical immunosensor for SARS-CoV-2 detection in saliva, by combining vertical and lateral flow. In detail, the device was constituted of a reservoir containing all reagents for the construction of the immunological chain onto the magnetic beads and a lateral flow holder which contained a polyester-based electrode, a magnet, and an adsorbent pad. The measurement was carried out by adding the saliva sample into the reservoir, followed by the addition of this solution in the hole present in the lateral flow holder. The successive additions of washing buffer and TMB solution in the lateral flow holder allowed the detection of N protein in saliva in the range of 0.06 to 4 mu g/mL with a detection limit equal to 30 ng/mL. The analysis of several saliva samples with the sensing tool and the reference method, demonstrated the effectiveness of this device, being able to identify positive patients with high values of CT e.g. 35. This new configuration paves the way for the realization of any magnetic beads-based immunosystem without waste solution production, enlarging the application of paper-based devices.

Fabiani, L., Fiore, L., Fillo, S., D'Amore, N., De Santis, R., Lista, F., et al. (2024). Smartphone-assisted paper-based electrochemical immunosensor for SARS-CoV-2 detection in saliva. BIOELECTROCHEMISTRY, 156 [10.1016/j.bioelechem.2023.108619].

Smartphone-assisted paper-based electrochemical immunosensor for SARS-CoV-2 detection in saliva

Fabiani L.;Fiore L.;Arduini F.
2024-01-01

Abstract

Herein, we developed a new waste solution-free paper-based electrochemical immunosensor for SARS-CoV-2 detection in saliva, by combining vertical and lateral flow. In detail, the device was constituted of a reservoir containing all reagents for the construction of the immunological chain onto the magnetic beads and a lateral flow holder which contained a polyester-based electrode, a magnet, and an adsorbent pad. The measurement was carried out by adding the saliva sample into the reservoir, followed by the addition of this solution in the hole present in the lateral flow holder. The successive additions of washing buffer and TMB solution in the lateral flow holder allowed the detection of N protein in saliva in the range of 0.06 to 4 mu g/mL with a detection limit equal to 30 ng/mL. The analysis of several saliva samples with the sensing tool and the reference method, demonstrated the effectiveness of this device, being able to identify positive patients with high values of CT e.g. 35. This new configuration paves the way for the realization of any magnetic beads-based immunosystem without waste solution production, enlarging the application of paper-based devices.
2024
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHEM-01/A - Chimica analitica
English
Senza Impact Factor ISI
Antigen detection
Carbon black
Paper-based fluidics
Polyester-based screen-printed electrodes
Fabiani, L., Fiore, L., Fillo, S., D'Amore, N., De Santis, R., Lista, F., et al. (2024). Smartphone-assisted paper-based electrochemical immunosensor for SARS-CoV-2 detection in saliva. BIOELECTROCHEMISTRY, 156 [10.1016/j.bioelechem.2023.108619].
Fabiani, L; Fiore, L; Fillo, S; D'Amore, N; De Santis, R; Lista, F; Arduini, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/388398
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