Sol-gel technique represents a remarkably versatile method for protein encapsulation. To enhance sol-gel biocompatibility, systems envisaging the presence of calcium and phosphates in the sol-gel composition were recently prepared and investigated. Unfortunately, the low pH at which solutions were prepared (pH < 2.5) dramatically limited their application to proteins, because the acidic environment induces protein denaturation. In this paper we apply a new protocol based on the introduction of calcium nitrate to the inorganic phase, with formation of a binary bioactive system. In this case protein encapsulation results versatile and secure, being achieved at a pH close to neutrality (pH 6.0); also, the presence of calcium is expected to enhance system biocompatibility. To determine the properties of the salt-doped sol-gel and the influence exerted on entrapped biosystems, the structural and functional properties of embedded cytochrome c have been investigated. Data obtained indicate that the salt-doped sol-gel induces no significant change in the structure and the redox properties of the embedded protein; also, the matrix increases protein stability. Interestingly, the presence of calcium nitrate appears determinant for refolding of the acid-denatured protein. This is of interest in the perspective of future applications in biosensoristic area.
Deriu D., P.S. (2008). Spectroscopic and electrochemical characterization of cytochrome c encapsulated in a bio sol-gel matrix. BIOMETALS, 21(4), 417-423 [10.1007/s10534-007-9130-7].
|Tipologia:||Articolo su rivista|
|Citazione:||Deriu D., P.S. (2008). Spectroscopic and electrochemical characterization of cytochrome c encapsulated in a bio sol-gel matrix. BIOMETALS, 21(4), 417-423 [10.1007/s10534-007-9130-7].|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/10|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s10534-007-9130-7|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2008|
|Titolo:||Spectroscopic and electrochemical characterization of cytochrome c encapsulated in a bio sol-gel matrix|
|Autori:||Deriu D., Pagnotta S.E., Santucci R., Rosato N.|
|Appare nelle tipologie:||01 - Articolo su rivista|