Peripheral blood mononuclear cells (PBMCs) from healthy individuals can be infected by human T-lymphotropic virus type 1 (HTLV-1) upon cocultivation of the PBMCs with irradiated HTLV-1-transformed human MT-2 cells. This model system closely mimics HTLV-1 transmission through cell-to-cell contact. Carbohydrate-binding agents (CBAs) such as the alpha(1,3)/alpha(1,6)mannose-specific Hippeastrum hybrid agglutinin and the GIcNAc-specific Urtica dioica agglutinin, and also the small, nonpeptidic alpha(1,2)-mannose-specific antibiotic pradimicin A, were able to efficiently prevent cell-to-cell HTLV-1 transmission at nontoxic concentrations, as evidenced by the lack of appearance of virus-specific mRNA and of the viral protein Tax in the acceptor cells. Consistently, antivirally active doses of CBAs fully prevented HTLV-1-induced stimulation of PBMC growth. The inhibitory effects of CBAs on HTLV-1 transmission were also evident when HTLV-1-infected C5MJ cells were used in place of MT-2 cells as a virus donor cell line. The anti-HTLV-1 properties of the CBAs highlight the importance of the envelope glycans in events underlying HTLV-1 passage from cell to cell and indicate that CBAs should be further investigated for their potential to prevent HTLV-1 infection, including mother-to-child virus transmission by cell-to-cell contact through breast milk feeding.
|Tipologia:||Articolo su rivista|
|Citazione:||Balestrieri, E., Ascolani, A., Igarashi, Y., Oki, T., Mastino, A., Balzarini, J., et al. (2008). Inhibition of cell-to-cell transmission of human T-cell lymphotropic virus type 1 in vitro by carbohydrate-binding agents. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 52(8), 2771-2779.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
Settore MED/07 - Microbiologia e Microbiologia Clinica
|Revisione (peer review):||Esperti anonimi|
|Digital Object Identifier (DOI):||10.1128/AAC.01671-07|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2008|
|Titolo:||Inhibition of cell-to-cell transmission of human T-cell lymphotropic virus type 1 in vitro by carbohydrate-binding agents|
|Autori interni:||BALESTRIERI, EMANUELA |
|Autori:||Balestrieri, E; Ascolani, A; Igarashi, Y; Oki, T; Mastino, A; Balzarini, J; Macchi, B|
|Appare nelle tipologie:||01 - Articolo su rivista|
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