far-infrared radiation (FIR) is emerging as a novel non-invasive tool for mitigating inflammation and oxidative stress, offering potential benefits for certain medical conditions such as cardiovascular disease and chronic inflammatory disorders. we previously demonstrated that the application of patch-based FIR therapy on human umbilical vein endothelial cells (HUVECs) reduced the expression of inflammatory biomarkers and the levels of reactive oxygen species (ROS). several in vitro studies have shown the inhibitory effects of FIR therapy on cell growth in different cancer cells (including murine melanoma cells), mainly using the wound healing assay, without direct cell motility or tracking analysis. the main objective of the present study was to conduct an in-depth analysis of single-cell motility and tracking during the wound healing assay, using an innovative high-throughput technique in the human melanoma cell line M14/C2. This technique evaluates various motility descriptors, such as average velocity, average curvature, average turning angle, and diffusion coefficient. our results demonstrated that patch-based FIR therapy did not impact cell proliferation and viability or the activation of mitogen-activated protein kinases (MAPKs) in the human melanoma cell line M14/C2. moreover, no significant differences in cell motility and tracking were observed between control cells and patch-treated cells. altogether, these findings confirm the beneficial effects of the in vitro application of patch-based FIR therapy in human melanoma cell lines, although such effects need to be confirmed in future in vivo studies.

Pacifici, F., Chiereghin, F., D'Orazio, M., Malatesta, G., Infante, M., Fazio, F., et al. (2024). Patch-Based Far-Infrared Radiation (FIR) Therapy Does Not Impact Cell Tracking or Motility of Human Melanoma Cells In Vitro. CURRENT ISSUES IN MOLECULAR BIOLOGY, 46(9), 10026-10037 [10.3390/cimb46090599].

Patch-Based Far-Infrared Radiation (FIR) Therapy Does Not Impact Cell Tracking or Motility of Human Melanoma Cells In Vitro

Francesca Pacifici;Francesca Chiereghin;Michele D'Orazio;Gina Malatesta;Marco Infante;Giulia Donadel;Eugenio Martinelli;Antonino De Lorenzo;David Della-Morte;Donatella Pastore
2024-01-01

Abstract

far-infrared radiation (FIR) is emerging as a novel non-invasive tool for mitigating inflammation and oxidative stress, offering potential benefits for certain medical conditions such as cardiovascular disease and chronic inflammatory disorders. we previously demonstrated that the application of patch-based FIR therapy on human umbilical vein endothelial cells (HUVECs) reduced the expression of inflammatory biomarkers and the levels of reactive oxygen species (ROS). several in vitro studies have shown the inhibitory effects of FIR therapy on cell growth in different cancer cells (including murine melanoma cells), mainly using the wound healing assay, without direct cell motility or tracking analysis. the main objective of the present study was to conduct an in-depth analysis of single-cell motility and tracking during the wound healing assay, using an innovative high-throughput technique in the human melanoma cell line M14/C2. This technique evaluates various motility descriptors, such as average velocity, average curvature, average turning angle, and diffusion coefficient. our results demonstrated that patch-based FIR therapy did not impact cell proliferation and viability or the activation of mitogen-activated protein kinases (MAPKs) in the human melanoma cell line M14/C2. moreover, no significant differences in cell motility and tracking were observed between control cells and patch-treated cells. altogether, these findings confirm the beneficial effects of the in vitro application of patch-based FIR therapy in human melanoma cell lines, although such effects need to be confirmed in future in vivo studies.
2024
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/46
Settore MEDS-26/A - Scienze tecniche di medicina di laboratorio
English
FIR
Far-Infrared Radiation
M14/C2 cell line
MAPKs
cell migration
patch-based FIR therapy
wound healing assay
Pacifici, F., Chiereghin, F., D'Orazio, M., Malatesta, G., Infante, M., Fazio, F., et al. (2024). Patch-Based Far-Infrared Radiation (FIR) Therapy Does Not Impact Cell Tracking or Motility of Human Melanoma Cells In Vitro. CURRENT ISSUES IN MOLECULAR BIOLOGY, 46(9), 10026-10037 [10.3390/cimb46090599].
Pacifici, F; Chiereghin, F; D'Orazio, M; Malatesta, G; Infante, M; Fazio, F; Bertinato, C; Donadel, G; Martinelli, E; DE LORENZO, A; Della-Morte, D; P...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/386323
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