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EnglishThe Escherichia coli gene aphA codes for a periplasmic acid phosphatase called AphA, belonging to class B bacterial phosphatases, which is part of the DDDD superfamily of phosphohydrolases. After our first report about its crystal structure, we have started a series of crystallographic studies aimed at understanding of the catalytic mechanism of the enzyme. Here, we report three crystal structures of the AphA enzyme in complex with the hydrolysis products of nucleoside monophosphate substrates and a fourth with a proposed intermediate analogue that appears to be covalently bound to the enzyme. Comparison with the native enzyme structure and with the available X-ray structures of different phosphatases provides clues about the enzyme chemistry and allows us to propose a catalytic mechanism for AphA, and to discuss it with respect to the mechanism of other bacterial and human phosphatases. (c) 2005 Elsevier Ltd. All rights reserved.
Calderone, V., Forleo, C., Benvenuti, M., Thaller, M.C., Rossolini, G.M., & Mangani, S. (2006). A structure-based proposal for the catalytic mechanism of the bacterial acid phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases. JOURNAL OF MOLECULAR BIOLOGY, 355(4), 708-721.
| Tipologia: | Articolo su rivista |
| Citazione: | Calderone, V., Forleo, C., Benvenuti, M., Thaller, M.C., Rossolini, G.M., & Mangani, S. (2006). A structure-based proposal for the catalytic mechanism of the bacterial acid phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases. JOURNAL OF MOLECULAR BIOLOGY, 355(4), 708-721. |
| IF: | Con Impact Factor ISI |
| Lingua: | English |
| Settore Scientifico Disciplinare: | Settore BIO/19 - Microbiologia Generale Settore MED/07 - Microbiologia e Microbiologia Clinica Settore CHIM/03 - Chimica Generale e Inorganica |
| Revisione (peer review): | Esperti anonimi |
| Tipo: | Articolo |
| Rilevanza: | Rilevanza internazionale |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.jmb.2005.10.068 |
| Stato di pubblicazione: | Pubblicato |
| Data di pubblicazione: | 2006 |
| Titolo: | A structure-based proposal for the catalytic mechanism of the bacterial acid phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases |
| Autori: | |
| Autori: | Calderone, V ; Forleo, C ; Benvenuti, M ; Thaller, MC ; Rossolini, GM ; Mangani, S |
| Appare nelle tipologie: | 01 - Articolo su rivista |
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| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| 2006 JMB structure based proposal.pdf | Articolo principale | N/A | Open Access Visualizza/Apri |
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