N-Acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides, a family of bioactive lipids including anandamide as the prototypical member. here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin layer chromatography (TLC).
Fezza, F., Mastrangelo, N., Maccarrone, M. (2023). Radiometric Assay of NAPE-PLD Activity. In Methods Mol Biol .. SpringerLink.
Radiometric Assay of NAPE-PLD Activity
Fezza F;Mastrangelo N;Maccarrone M.
2023-09-25
Abstract
N-Acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides, a family of bioactive lipids including anandamide as the prototypical member. here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin layer chromatography (TLC).File in questo prodotto:
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