A cDNA vaccine (pVax1/pet-neu) was designed to encode 12 different Her-2/ErbB-2-derived, HLA-A*0201-restricted dominant and high-affinity heteroclitic cryptic epitopes. Vaccination with pVax1/pet-neu triggered multiple and ErbB-2-specific CTL responses in HLA-A*0201 transgenic HHD mice and in HLA-A*0201 healthy donors in vitro. Human and murine CTL specific for each one of the 12 ErbB-2 peptides recognized in vitro both human and murine tumor cells overexpressing endogenous ErbB-2. Furthermore, vaccination of HHD mice with pVax1/pet-neu significantly delayed the in vivo growth of challenged ErbB-2-expressing tumor (EL4/HHD/neu murine thymoma) more actively when compared with vaccination with the empty vector (pVax1) or vehicle alone. These data indicate that the pVax1/pet-neu cDNA vaccine coding for a poly-ErbB-2 epitope is able to generate simultaneous ErbB-2-specific antitumor responses against dominant and cryptic multiple epitopes.
Scardino, A., Alimandi, M., Correale, P., Smith, S.G., Bei, R., Firat, H., et al. (2007). A polyepitope DNA vaccine targeted to Her-2/ErbB-2 elicits a broad range of human and murine CTL effectors to protect against tumor challenge. CANCER RESEARCH, 67(14), 7028-7036.
Tipologia: | Articolo su rivista |
Citazione: | Scardino, A., Alimandi, M., Correale, P., Smith, S.G., Bei, R., Firat, H., et al. (2007). A polyepitope DNA vaccine targeted to Her-2/ErbB-2 elicits a broad range of human and murine CTL effectors to protect against tumor challenge. CANCER RESEARCH, 67(14), 7028-7036. |
IF: | Con Impact Factor ISI |
Lingua: | English |
Settore Scientifico Disciplinare: | Settore MED/04 - Patologia Generale |
Revisione (peer review): | Sì, ma tipo non specificato |
Tipo: | Articolo |
Rilevanza: | Rilevanza internazionale |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1158/0008-5472.CAN-06-3998 |
Stato di pubblicazione: | Pubblicato |
Data di pubblicazione: | 2007 |
Titolo: | A polyepitope DNA vaccine targeted to Her-2/ErbB-2 elicits a broad range of human and murine CTL effectors to protect against tumor challenge |
Autori: | |
Autori: | Scardino, A; Alimandi, M; Correale, P; Smith, SG; Bei, R; Firat, H; Cusi, MG; Faure, O; Graf-Dubois, S; Cencioni, G; Marrocco, J; Chouaib, S; Lemonnier, FA; Jackson, AM; Kosmatopoulos, K |
Appare nelle tipologie: | 01 - Articolo su rivista |