A cDNA vaccine (pVax1/pet-neu) was designed to encode 12 different Her-2/ErbB-2-derived, HLA-A*0201-restricted dominant and high-affinity heteroclitic cryptic epitopes. Vaccination with pVax1/pet-neu triggered multiple and ErbB-2-specific CTL responses in HLA-A*0201 transgenic HHD mice and in HLA-A*0201 healthy donors in vitro. Human and murine CTL specific for each one of the 12 ErbB-2 peptides recognized in vitro both human and murine tumor cells overexpressing endogenous ErbB-2. Furthermore, vaccination of HHD mice with pVax1/pet-neu significantly delayed the in vivo growth of challenged ErbB-2-expressing tumor (EL4/HHD/neu murine thymoma) more actively when compared with vaccination with the empty vector (pVax1) or vehicle alone. These data indicate that the pVax1/pet-neu cDNA vaccine coding for a poly-ErbB-2 epitope is able to generate simultaneous ErbB-2-specific antitumor responses against dominant and cryptic multiple epitopes.

Scardino, A., Alimandi, M., Correale, P., Smith, S.G., Bei, R., Firat, H., et al. (2007). A polyepitope DNA vaccine targeted to Her-2/ErbB-2 elicits a broad range of human and murine CTL effectors to protect against tumor challenge. CANCER RESEARCH, 67(14), 7028-7036 [10.1158/0008-5472.CAN-06-3998].

A polyepitope DNA vaccine targeted to Her-2/ErbB-2 elicits a broad range of human and murine CTL effectors to protect against tumor challenge

BEI, ROBERTO;
2007

Abstract

A cDNA vaccine (pVax1/pet-neu) was designed to encode 12 different Her-2/ErbB-2-derived, HLA-A*0201-restricted dominant and high-affinity heteroclitic cryptic epitopes. Vaccination with pVax1/pet-neu triggered multiple and ErbB-2-specific CTL responses in HLA-A*0201 transgenic HHD mice and in HLA-A*0201 healthy donors in vitro. Human and murine CTL specific for each one of the 12 ErbB-2 peptides recognized in vitro both human and murine tumor cells overexpressing endogenous ErbB-2. Furthermore, vaccination of HHD mice with pVax1/pet-neu significantly delayed the in vivo growth of challenged ErbB-2-expressing tumor (EL4/HHD/neu murine thymoma) more actively when compared with vaccination with the empty vector (pVax1) or vehicle alone. These data indicate that the pVax1/pet-neu cDNA vaccine coding for a poly-ErbB-2 epitope is able to generate simultaneous ErbB-2-specific antitumor responses against dominant and cryptic multiple epitopes.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/04 - Patologia Generale
eng
Con Impact Factor ISI
complementary DNA; DNA vaccine; drug vehicle; epidermal growth factor receptor 2; HLA A antigen; plasmid vector; protein vax1 pet neu; animal cell; antineoplastic activity; article; binding affinity; cancer immunization; cancer prevention; controlled study; cytotoxic T lymphocyte; donor; drug binding; drug cytotoxicity; drug targeting; gene overexpression; genetic transfection; human; human cell; immunogenicity; in vitro study; in vivo study; molecular recognition; mouse; nonhuman; oncogene neu; priority journal; provocation test; thymoma; transgenic mouse; tumor cell; tumor growth; tumor immunity; tumor volume; vaccine production; Animals; Cancer Vaccines; Cell Line, Tumor; Epitopes; Humans; Mice; Mice, Transgenic; Models, Genetic; Neoplasm Transplantation; Neoplasms; Peptides; Receptor, erbB-2; T-Lymphocytes, Cytotoxic; Thymoma; Vaccines, DNA
Scardino, A., Alimandi, M., Correale, P., Smith, S.G., Bei, R., Firat, H., et al. (2007). A polyepitope DNA vaccine targeted to Her-2/ErbB-2 elicits a broad range of human and murine CTL effectors to protect against tumor challenge. CANCER RESEARCH, 67(14), 7028-7036 [10.1158/0008-5472.CAN-06-3998].
Scardino, A; Alimandi, M; Correale, P; Smith, S; Bei, R; Firat, H; Cusi, M; Faure, O; Graf Dubois, S; Cencioni, G; Marrocco, J; Chouaib, S; Lemonnier, F; Jackson, A; Kosmatopoulos, K
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/36562
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