Dendritic cells (DCs) are thought to play a key role in driving the immunopathogenic response underlying chronic inflammatory arthritis. In this study, we have examined the presence and phenotype of plasmacytoid DCs (pDCs) in the synovial fluids (SF) of patients with rheumatoid arthritis (RA),,psoriatic arthritis (PA), and osteoarthritis (OA) and determined the chemotactic properties of SF from these patients toward pDCs. Flow cytometry analysis showed that the percentage of pDCs, identified as a population of Lin(-)CD123(++) cells, is 4- to 5-fold higher in RA SF and PA SIT than in OA SF. The morphological and immunophenotypic characterization of pDCs isolated from PA and RA SF indicates that they are in an immature state, most likely due to inhibitory factors present in RA SF, but are still able to undergo maturation when exposed ex vivo to viral agent or unmethylated DNA. CD123(+) and BDCA2(+) pDCs were detected by immunohistochemistry in RA synovial tissue in which expression of the IFN-alpha-inducible protein MxA was also found, suggesting production of type I IFN by maturing pDCs. We also show that CXCR3 and CXCR4 are expressed by both blood-derived pDCs and pDCs isolated from RA and PA SF and that CXCL-10, CXCL-11, and CXCL-12 present in RA and PA SIT stimulate chemotaxis of blood-derived pDCs. Altogether, these findings suggest that chemokine-driven recruitment of pDCs from the blood to the inflamed synovium could be important in the regulation of the immune response in chronic inflammatory arthritis.

Lande, R., Giacomini, E., Serafini, B., Rosicarelli, B., Sebastiani, G.d., Minisola, G., et al. (2004). Characterization and recruitment of plasmacytoid dendritic cells in synovial fluid and tissue of patients with chronic inflammatory arthritis. JOURNAL OF IMMUNOLOGY, 173(4), 2815-2824.

Characterization and recruitment of plasmacytoid dendritic cells in synovial fluid and tissue of patients with chronic inflammatory arthritis

TARANTINO, UMBERTO;
2004-01-01

Abstract

Dendritic cells (DCs) are thought to play a key role in driving the immunopathogenic response underlying chronic inflammatory arthritis. In this study, we have examined the presence and phenotype of plasmacytoid DCs (pDCs) in the synovial fluids (SF) of patients with rheumatoid arthritis (RA),,psoriatic arthritis (PA), and osteoarthritis (OA) and determined the chemotactic properties of SF from these patients toward pDCs. Flow cytometry analysis showed that the percentage of pDCs, identified as a population of Lin(-)CD123(++) cells, is 4- to 5-fold higher in RA SF and PA SIT than in OA SF. The morphological and immunophenotypic characterization of pDCs isolated from PA and RA SF indicates that they are in an immature state, most likely due to inhibitory factors present in RA SF, but are still able to undergo maturation when exposed ex vivo to viral agent or unmethylated DNA. CD123(+) and BDCA2(+) pDCs were detected by immunohistochemistry in RA synovial tissue in which expression of the IFN-alpha-inducible protein MxA was also found, suggesting production of type I IFN by maturing pDCs. We also show that CXCR3 and CXCR4 are expressed by both blood-derived pDCs and pDCs isolated from RA and PA SF and that CXCL-10, CXCL-11, and CXCL-12 present in RA and PA SIT stimulate chemotaxis of blood-derived pDCs. Altogether, these findings suggest that chemokine-driven recruitment of pDCs from the blood to the inflamed synovium could be important in the regulation of the immune response in chronic inflammatory arthritis.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/33 - Malattie Apparato Locomotore
Italian
alpha interferon; CD123 antigen; chemokine; chemokine receptor CXCR3; chemokine receptor CXCR4; CXCL11 chemokine; gamma interferon inducible protein 10; Myxovirus resistance protein A; stromal cell derived factor 1; unclassified drug; adult; aged; article; cell isolation; cell maturation; chemotaxis; chronic arthritis; clinical article; controlled study; dendritic cell; female; flow cytometry; human; human cell; immune response; immunohistochemistry; immunopathogenesis; immunophenotyping; immunoregulation; interferon production; male; osteoarthritis; phenotype; priority journal; protein expression; psoriatic arthritis; rheumatoid arthritis; synovial fluid; Antigens, Surface; Arthritis; Cell Differentiation; Chemokines, CXC; Chemotaxis, Leukocyte; Chronic Disease; Dendritic Cells; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Humans; Immunohistochemistry; Immunophenotyping; Interferon Type I; Interleukin-3 Receptor alpha Subunit; Phenotype; Receptors, Chemokine; Receptors, CXCR4; Receptors, Interleukin-3; Synovial Fluid; Synovial Membrane
PMID: 15295000
Lande, R., Giacomini, E., Serafini, B., Rosicarelli, B., Sebastiani, G.d., Minisola, G., et al. (2004). Characterization and recruitment of plasmacytoid dendritic cells in synovial fluid and tissue of patients with chronic inflammatory arthritis. JOURNAL OF IMMUNOLOGY, 173(4), 2815-2824.
Lande, R; Giacomini, E; Serafini, B; Rosicarelli, B; Sebastiani, Gd; Minisola, G; Tarantino, U; Riccieri, V; Valesini, G; Coccia, Em
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/36473
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