3D Cell Migration Chip (3DCM‐Chip): a new tool toward the modeling of 3D cellular complex systems

3D hydrogel-based cell cultures provide models for studying cell behavior and can efficiently replicate the physiologic environment. Hydrogels can be tailored to mimic mechanical and biochemical properties of specific tissues and allow to produce gel-in-gel models. In this system, microspheres encapsulating cells are embedded in an outer hydrogel matrix, where cells are able to migrate. To enhance the efficiency of such studies, we design a lab-on-a-chip named 3D Cell Migration-chip (3DCM-chip) that offers substantial advantages over traditional methods. 3DCM-chip facilitates the analysis of biochemical and physical stimuli effects on cell migration/invasion in different cell types, including stem, normal and tumor cells. 3DCM-chip provides a smart platform for developing more complex cell co-cultures systems. Herein we investigate the impact of human fibroblasts on MDA-MB 231 breast cancer cells invasiveness. Moreover, how the presence of different cellular lines, including mesenchymal stem cells (hMSCs), normal human dermal fibroblasts (NHDFs), and human umbilical vein endothelial cells (HUVECs), affects the invasive behavior of cancer cells is investigated using 3DCM-chip and producing predictive tumoroid models with a more complex network of interactions between cells and microenvironment. 3DCM-chip moves us closer to creating in vitro systems that can potentially replicate key aspects of the physiological tumor microenvironment.